Immune responses to Streptococcus sobrinus surface protein antigen A expressed by recombinant Salmonella typhimurium

T. A. Doggett, E. K. Jagusztyn-Krynicka, R. Curtiss

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study, we used a vaccine strain of Salmonella typhimurium to express antigenic determinants of the SpaA antigen of Streptococcus sobrinus, which is involved in the caries-forming process. We cloned either a single repeat (pYA2901) or three tandem repeats (pYA2905) of the 0.48-kb fragment of the spaA gene, which codes for an important component of the SpaA protein, plus a 1.2-kb minor antigenic determinant and measured the resulting immune responses to SpaA in orally immunized BALB/c mice. The single or triple repeat of the spaA gene fragment was inserted into the Asd+ vector pYA292 and was transformed into the S. typhimurium Δcya Δcrp vaccine strain χ4072 containing Δasd in the chromosome. Female BALB/c mice were then orally immunized with two doses of the S. typhimurium containing either of the two SpaA constructs, and the immune responses to the expressed SpaA protein were assessed. Significant serum immunoglobulin G (IgG) anti-SpaA titers were detected in mice immunized with χ4072(pYA2905) but not χ4072(pYA2901). Salivary anti-SpaA IgA titers were minimal and were only detected in mice immunized with S. typhimurium expressing the SpaA encoded by pYA2905. Intestinal anti-SpaA IgA titers, however, were detected in both groups of mice, particularly in mice immunized with χ4072(pYA2905). An oral booster 26 weeks after the initial series of immunizations resulted in increased serum IgG titers in both χ4072(pYA2901)- and χ4072(pYA2905)-immunized animals, particularly in the χ4072(pYA2905)-immunized animals. No anamnestic IgA response was detected in the saliva following the booster immunization.

Original languageEnglish (US)
Pages (from-to)1859-1866
Number of pages8
JournalInfection and Immunity
Volume61
Issue number5
StatePublished - 1993
Externally publishedYes

Fingerprint

Streptococcus sobrinus
Salmonella typhimurium
Surface Antigens
Membrane Proteins
Epitopes
Vaccines
Immunoglobulin G
Secondary Immunization
Tandem Repeat Sequences
Serum
Saliva
Immunoglobulin A
Genes
Immunization
Proteins
Chromosomes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Immune responses to Streptococcus sobrinus surface protein antigen A expressed by recombinant Salmonella typhimurium. / Doggett, T. A.; Jagusztyn-Krynicka, E. K.; Curtiss, R.

In: Infection and Immunity, Vol. 61, No. 5, 1993, p. 1859-1866.

Research output: Contribution to journalArticle

Doggett, T. A. ; Jagusztyn-Krynicka, E. K. ; Curtiss, R. / Immune responses to Streptococcus sobrinus surface protein antigen A expressed by recombinant Salmonella typhimurium. In: Infection and Immunity. 1993 ; Vol. 61, No. 5. pp. 1859-1866.
@article{9b40d7144c11495fa4b25b2394800e9a,
title = "Immune responses to Streptococcus sobrinus surface protein antigen A expressed by recombinant Salmonella typhimurium",
abstract = "In this study, we used a vaccine strain of Salmonella typhimurium to express antigenic determinants of the SpaA antigen of Streptococcus sobrinus, which is involved in the caries-forming process. We cloned either a single repeat (pYA2901) or three tandem repeats (pYA2905) of the 0.48-kb fragment of the spaA gene, which codes for an important component of the SpaA protein, plus a 1.2-kb minor antigenic determinant and measured the resulting immune responses to SpaA in orally immunized BALB/c mice. The single or triple repeat of the spaA gene fragment was inserted into the Asd+ vector pYA292 and was transformed into the S. typhimurium Δcya Δcrp vaccine strain χ4072 containing Δasd in the chromosome. Female BALB/c mice were then orally immunized with two doses of the S. typhimurium containing either of the two SpaA constructs, and the immune responses to the expressed SpaA protein were assessed. Significant serum immunoglobulin G (IgG) anti-SpaA titers were detected in mice immunized with χ4072(pYA2905) but not χ4072(pYA2901). Salivary anti-SpaA IgA titers were minimal and were only detected in mice immunized with S. typhimurium expressing the SpaA encoded by pYA2905. Intestinal anti-SpaA IgA titers, however, were detected in both groups of mice, particularly in mice immunized with χ4072(pYA2905). An oral booster 26 weeks after the initial series of immunizations resulted in increased serum IgG titers in both χ4072(pYA2901)- and χ4072(pYA2905)-immunized animals, particularly in the χ4072(pYA2905)-immunized animals. No anamnestic IgA response was detected in the saliva following the booster immunization.",
author = "Doggett, {T. A.} and Jagusztyn-Krynicka, {E. K.} and R. Curtiss",
year = "1993",
language = "English (US)",
volume = "61",
pages = "1859--1866",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Immune responses to Streptococcus sobrinus surface protein antigen A expressed by recombinant Salmonella typhimurium

AU - Doggett, T. A.

AU - Jagusztyn-Krynicka, E. K.

AU - Curtiss, R.

PY - 1993

Y1 - 1993

N2 - In this study, we used a vaccine strain of Salmonella typhimurium to express antigenic determinants of the SpaA antigen of Streptococcus sobrinus, which is involved in the caries-forming process. We cloned either a single repeat (pYA2901) or three tandem repeats (pYA2905) of the 0.48-kb fragment of the spaA gene, which codes for an important component of the SpaA protein, plus a 1.2-kb minor antigenic determinant and measured the resulting immune responses to SpaA in orally immunized BALB/c mice. The single or triple repeat of the spaA gene fragment was inserted into the Asd+ vector pYA292 and was transformed into the S. typhimurium Δcya Δcrp vaccine strain χ4072 containing Δasd in the chromosome. Female BALB/c mice were then orally immunized with two doses of the S. typhimurium containing either of the two SpaA constructs, and the immune responses to the expressed SpaA protein were assessed. Significant serum immunoglobulin G (IgG) anti-SpaA titers were detected in mice immunized with χ4072(pYA2905) but not χ4072(pYA2901). Salivary anti-SpaA IgA titers were minimal and were only detected in mice immunized with S. typhimurium expressing the SpaA encoded by pYA2905. Intestinal anti-SpaA IgA titers, however, were detected in both groups of mice, particularly in mice immunized with χ4072(pYA2905). An oral booster 26 weeks after the initial series of immunizations resulted in increased serum IgG titers in both χ4072(pYA2901)- and χ4072(pYA2905)-immunized animals, particularly in the χ4072(pYA2905)-immunized animals. No anamnestic IgA response was detected in the saliva following the booster immunization.

AB - In this study, we used a vaccine strain of Salmonella typhimurium to express antigenic determinants of the SpaA antigen of Streptococcus sobrinus, which is involved in the caries-forming process. We cloned either a single repeat (pYA2901) or three tandem repeats (pYA2905) of the 0.48-kb fragment of the spaA gene, which codes for an important component of the SpaA protein, plus a 1.2-kb minor antigenic determinant and measured the resulting immune responses to SpaA in orally immunized BALB/c mice. The single or triple repeat of the spaA gene fragment was inserted into the Asd+ vector pYA292 and was transformed into the S. typhimurium Δcya Δcrp vaccine strain χ4072 containing Δasd in the chromosome. Female BALB/c mice were then orally immunized with two doses of the S. typhimurium containing either of the two SpaA constructs, and the immune responses to the expressed SpaA protein were assessed. Significant serum immunoglobulin G (IgG) anti-SpaA titers were detected in mice immunized with χ4072(pYA2905) but not χ4072(pYA2901). Salivary anti-SpaA IgA titers were minimal and were only detected in mice immunized with S. typhimurium expressing the SpaA encoded by pYA2905. Intestinal anti-SpaA IgA titers, however, were detected in both groups of mice, particularly in mice immunized with χ4072(pYA2905). An oral booster 26 weeks after the initial series of immunizations resulted in increased serum IgG titers in both χ4072(pYA2901)- and χ4072(pYA2905)-immunized animals, particularly in the χ4072(pYA2905)-immunized animals. No anamnestic IgA response was detected in the saliva following the booster immunization.

UR - http://www.scopus.com/inward/record.url?scp=0027173915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027173915&partnerID=8YFLogxK

M3 - Article

C2 - 8478075

AN - SCOPUS:0027173915

VL - 61

SP - 1859

EP - 1866

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -