Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization

Ho Young Kang, Roy Curtiss

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His6-tagged rPspA (His6-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 109 colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×107) was 104 times higher than induced by Salmonella carrying pYA3496 (<2.4×103).

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume37
Issue number2-3
DOIs
StatePublished - Jul 15 2003

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Keywords

  • Immune response
  • PspA antigen
  • Salmonella vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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