Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization

Ho Young Kang; Roy Curtiss

doi:10.1016/S0928-8244(03)00063-4

Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization

Ho Young Kang, Roy Curtiss

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His₆-tagged rPspA (His₆-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 10⁹ colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×10⁷) was 10⁴ times higher than induced by Salmonella carrying pYA3496 (<2.4×10³).

Original language	English (US)
Pages (from-to)	99-104
Number of pages	6
Journal	FEMS Immunology and Medical Microbiology
Volume	37
Issue number	2-3
DOIs	https://doi.org/10.1016/S0928-8244(03)00063-4
State	Published - Jul 15 2003
Externally published	Yes

Keywords

Immune response
PspA antigen
Salmonella vaccine

ASJC Scopus subject areas

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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10.1016/S0928-8244(03)00063-4

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title = "Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization",

abstract = "The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His6-tagged rPspA (His6-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 109 colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×107) was 104 times higher than induced by Salmonella carrying pYA3496 (<2.4×103).",

keywords = "Immune response, PspA antigen, Salmonella vaccine",

author = "Kang, {Ho Young} and Roy Curtiss",

note = "Funding Information: This research project was supported by a grant from the U.S. Public Health Service through the National Institutes of Health (DE06669).",

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AU - Curtiss, Roy

N1 - Funding Information: This research project was supported by a grant from the U.S. Public Health Service through the National Institutes of Health (DE06669).

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N2 - The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His6-tagged rPspA (His6-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 109 colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×107) was 104 times higher than induced by Salmonella carrying pYA3496 (<2.4×103).

AB - The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His6-tagged rPspA (His6-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 109 colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×107) was 104 times higher than induced by Salmonella carrying pYA3496 (<2.4×103).

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Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization

Abstract

Keywords

ASJC Scopus subject areas

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