Immobilization and characterization of γ-Aminobutyric acid on gold surface

Tingting Wang, Gholam Ehteshami, Stephen Massia, Jitendran Muthuswamy

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

γ-Aminobutyric acid (GABA) is one of two main inhibitory neurotransmitters in the central nervous system that plays an important role in neuronal function and dysfunction. Immobilization of GABA molecules on a rigid surface in an ordered fashion will provide an opportunity to understand some of the fundamental properties related to its structure and function. In this study, we report a novel strategy for immobilization of bioactive GABA on gold substrate. GABA was immobilized in three consecutive steps, namely gold substrate amination, dextran covalent attachment, and GABA immobilization. Surface chemistry was verified at each step using XPS and FTIR. Bioactivity of GABA immobilized on the gold surface was studied using atomic force microscopy to reveal antigen-antibody binding. Nonspecific protein adsorption on the bioactive surface was analyzed quantitatively using anti-GABA antibody and an enzyme linked nonspecific anti-immunoglobulin-G antibody in an ELISA assay. GABA functionalized surface has high affinity for anti-GABA, while showing significantly low affinity for non-specific anti-IgG antibody. All these data support the presence of a bio-functional immobilized GABA on the gold surface. In conclusion, we report a novel technique for immobilizing bioactive GABA molecules in an orderly fashion on gold substrates.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume79
Issue number1
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Bioactive surface
  • Dextran
  • Functionalization
  • Gold substrate
  • Neurotransmitter

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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