Immimohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis

T. L. Pertile, M. M. Walser, J. M. Sharma, J. L. Shivers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We characterized the lymphocytes in the tarsal joint synovium of chickens inoculated with an arthrotropic strain of avian reovirus. Cryostat sections of whole joints taken from 2 days to 35 days postinoculation were analyzed using monoclonal antibodies directed against B lymphocytes, T lymphocytes, and chicken Ia antigen. Plasma cells were morphologically identified using stained sections of whole joints. Time-dependent changes were found in the type and number of positively staining cells. Synoviocytes and cells with a dendritic morphology stained positive for Ia in normal joint sections. T cells, mostly CD8 positive, were present in low numbers in acute phase arthritis (2-6 days postinfection) in the perivascular and superficial regions of the synovium. Subacute arthritis (8-14 days postinfection) was characterized by increased numbers of CD4 and CD8 T cells in the perivascular and superficial regions. The perivascular T cells began to organize into aggregates, with IgM-positive B cells and plasma cells on the periphery of these aggregates. Some CD8-positive cells were detected on the surface of the articular cartilage. Cells staining positively for Ia were not lymphocytes. Chronic arthritis (> 14 days postinfection) was characterized by large numbers of T cells in the perivascular and superficial regions, with the CD4-positive T cells found primarly in the lymphoid aggregates of the perivascular regions. IgM-positive B cells were fewer, but more plasma cells, few of which stained positive for IgM, were present. Lymphocytes in chronic arthritis stained positively for Ia. These data suggest that the types, numbers, and activation level of lymphocytes present in the tarsal joints are similar but not identical to those seen in rheumatoid arthritis.

Original languageEnglish (US)
Pages (from-to)303-310
Number of pages8
JournalVeterinary Pathology
Volume33
Issue number3
StatePublished - May 1996
Externally publishedYes

Fingerprint

Tarsal Joints
Infectious Arthritis
Experimental Arthritis
Lymphocyte Subsets
arthritis
joints (animal)
Chickens
lymphocytes
T-lymphocytes
chickens
T-Lymphocytes
Arthritis
plasma cells
cell aggregates
Plasma Cells
B-lymphocytes
Immunoglobulin M
B-Lymphocytes
Synovial Membrane
Joints

Keywords

  • Chickens immunohistochemistry
  • Light microscopy
  • Reovirus
  • Tarsal joint
  • Viral arthritis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Immimohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis. / Pertile, T. L.; Walser, M. M.; Sharma, J. M.; Shivers, J. L.

In: Veterinary Pathology, Vol. 33, No. 3, 05.1996, p. 303-310.

Research output: Contribution to journalArticle

Pertile, T. L. ; Walser, M. M. ; Sharma, J. M. ; Shivers, J. L. / Immimohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis. In: Veterinary Pathology. 1996 ; Vol. 33, No. 3. pp. 303-310.
@article{f6bca8156dd348a4b6db34a6f7a62b9a,
title = "Immimohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis",
abstract = "We characterized the lymphocytes in the tarsal joint synovium of chickens inoculated with an arthrotropic strain of avian reovirus. Cryostat sections of whole joints taken from 2 days to 35 days postinoculation were analyzed using monoclonal antibodies directed against B lymphocytes, T lymphocytes, and chicken Ia antigen. Plasma cells were morphologically identified using stained sections of whole joints. Time-dependent changes were found in the type and number of positively staining cells. Synoviocytes and cells with a dendritic morphology stained positive for Ia in normal joint sections. T cells, mostly CD8 positive, were present in low numbers in acute phase arthritis (2-6 days postinfection) in the perivascular and superficial regions of the synovium. Subacute arthritis (8-14 days postinfection) was characterized by increased numbers of CD4 and CD8 T cells in the perivascular and superficial regions. The perivascular T cells began to organize into aggregates, with IgM-positive B cells and plasma cells on the periphery of these aggregates. Some CD8-positive cells were detected on the surface of the articular cartilage. Cells staining positively for Ia were not lymphocytes. Chronic arthritis (> 14 days postinfection) was characterized by large numbers of T cells in the perivascular and superficial regions, with the CD4-positive T cells found primarly in the lymphoid aggregates of the perivascular regions. IgM-positive B cells were fewer, but more plasma cells, few of which stained positive for IgM, were present. Lymphocytes in chronic arthritis stained positively for Ia. These data suggest that the types, numbers, and activation level of lymphocytes present in the tarsal joints are similar but not identical to those seen in rheumatoid arthritis.",
keywords = "Chickens immunohistochemistry, Light microscopy, Reovirus, Tarsal joint, Viral arthritis",
author = "Pertile, {T. L.} and Walser, {M. M.} and Sharma, {J. M.} and Shivers, {J. L.}",
year = "1996",
month = "5",
language = "English (US)",
volume = "33",
pages = "303--310",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Immimohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis

AU - Pertile, T. L.

AU - Walser, M. M.

AU - Sharma, J. M.

AU - Shivers, J. L.

PY - 1996/5

Y1 - 1996/5

N2 - We characterized the lymphocytes in the tarsal joint synovium of chickens inoculated with an arthrotropic strain of avian reovirus. Cryostat sections of whole joints taken from 2 days to 35 days postinoculation were analyzed using monoclonal antibodies directed against B lymphocytes, T lymphocytes, and chicken Ia antigen. Plasma cells were morphologically identified using stained sections of whole joints. Time-dependent changes were found in the type and number of positively staining cells. Synoviocytes and cells with a dendritic morphology stained positive for Ia in normal joint sections. T cells, mostly CD8 positive, were present in low numbers in acute phase arthritis (2-6 days postinfection) in the perivascular and superficial regions of the synovium. Subacute arthritis (8-14 days postinfection) was characterized by increased numbers of CD4 and CD8 T cells in the perivascular and superficial regions. The perivascular T cells began to organize into aggregates, with IgM-positive B cells and plasma cells on the periphery of these aggregates. Some CD8-positive cells were detected on the surface of the articular cartilage. Cells staining positively for Ia were not lymphocytes. Chronic arthritis (> 14 days postinfection) was characterized by large numbers of T cells in the perivascular and superficial regions, with the CD4-positive T cells found primarly in the lymphoid aggregates of the perivascular regions. IgM-positive B cells were fewer, but more plasma cells, few of which stained positive for IgM, were present. Lymphocytes in chronic arthritis stained positively for Ia. These data suggest that the types, numbers, and activation level of lymphocytes present in the tarsal joints are similar but not identical to those seen in rheumatoid arthritis.

AB - We characterized the lymphocytes in the tarsal joint synovium of chickens inoculated with an arthrotropic strain of avian reovirus. Cryostat sections of whole joints taken from 2 days to 35 days postinoculation were analyzed using monoclonal antibodies directed against B lymphocytes, T lymphocytes, and chicken Ia antigen. Plasma cells were morphologically identified using stained sections of whole joints. Time-dependent changes were found in the type and number of positively staining cells. Synoviocytes and cells with a dendritic morphology stained positive for Ia in normal joint sections. T cells, mostly CD8 positive, were present in low numbers in acute phase arthritis (2-6 days postinfection) in the perivascular and superficial regions of the synovium. Subacute arthritis (8-14 days postinfection) was characterized by increased numbers of CD4 and CD8 T cells in the perivascular and superficial regions. The perivascular T cells began to organize into aggregates, with IgM-positive B cells and plasma cells on the periphery of these aggregates. Some CD8-positive cells were detected on the surface of the articular cartilage. Cells staining positively for Ia were not lymphocytes. Chronic arthritis (> 14 days postinfection) was characterized by large numbers of T cells in the perivascular and superficial regions, with the CD4-positive T cells found primarly in the lymphoid aggregates of the perivascular regions. IgM-positive B cells were fewer, but more plasma cells, few of which stained positive for IgM, were present. Lymphocytes in chronic arthritis stained positively for Ia. These data suggest that the types, numbers, and activation level of lymphocytes present in the tarsal joints are similar but not identical to those seen in rheumatoid arthritis.

KW - Chickens immunohistochemistry

KW - Light microscopy

KW - Reovirus

KW - Tarsal joint

KW - Viral arthritis

UR - http://www.scopus.com/inward/record.url?scp=0030144115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030144115&partnerID=8YFLogxK

M3 - Article

C2 - 8740704

AN - SCOPUS:0030144115

VL - 33

SP - 303

EP - 310

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 3

ER -