Imaging DNA molecules chemically bound to a mica surface (Invited Paper)

Stuart Lindsay, Y. L. Lyubchenko, A. A. Gall, L. S. Shlyakhtenko, R. E. Harrington

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Scopus citations

Abstract

We describe a procedure for imaging DNA molecules using atomic force microscopy (AFM). Double-stranded DNA binds to a mica surface modified by treatment with 3- aminopropyltriethoxysilane to yield AFM images that are stable under repeated scanning. We used this procedure to obtain high quality images of unstained linear DNA molecules and circular DNA molecules. The resolution was limited to tens of nanometers by the profile of the AFM tips.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Place of PublicationBellingham, WA, United States
PublisherPubl by Int Soc for Optical Engineering
Pages84-90
Number of pages7
Volume1639
ISBN (Print)0819407852
Publication statusPublished - 1992
EventScanning Probe Microscopies - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 22 1992

Other

OtherScanning Probe Microscopies
CityLos Angeles, CA, USA
Period1/20/921/22/92

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Lindsay, S., Lyubchenko, Y. L., Gall, A. A., Shlyakhtenko, L. S., & Harrington, R. E. (1992). Imaging DNA molecules chemically bound to a mica surface (Invited Paper). In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1639, pp. 84-90). Bellingham, WA, United States: Publ by Int Soc for Optical Engineering.