Abstract
2-Fluoro-4-nitrophenol-β-d-galactopyranoside (OFPNPG) belongs to a novel class of NMR active molecules (fluoroaryl-β-d-galactopyranosides), which are highly responsive to the action of β-galactosidase (β-gal). OFPNPG has a single 19F peak (-55 ppm relative to aqueous sodium trifluoroacetate). Upon cleavage by β-gal, the pH sensitive aglycone 2-fluoro-4-nitrophenol (OFPNP) is observed at a chemical shift of -59 to -61 ppm. The chemical shift response is sufficient to observe β-gal activity using chemical shift imaging (CSI). 19F CSI studies of enzyme activity and lacZ gene expression in 9L-glioma and MCF7 breast cancer cells are presented, providing further evidence for the utility of OFPNPG as a gene-reporter molecule for future in vivo studies.
Original language | English (US) |
---|---|
Pages (from-to) | 959-962 |
Number of pages | 4 |
Journal | Magnetic Resonance Imaging |
Volume | 24 |
Issue number | 7 |
DOIs | |
State | Published - Sep 2006 |
Externally published | Yes |
Keywords
- Breast cancer
- CSI
- F NMR
- Phenylgalactopyranosides
- lacZ gene reporter
- β-Galactosidase
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Radiology Nuclear Medicine and imaging