Identity of the Photosystem II reaction center polypeptide

H. Y. Nakatani, B. Ke, E. Dolan, C. J. Arntzen

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalBBA - Bioenergetics
Volume765
Issue number3
DOIs
StatePublished - Jun 26 1984

Keywords

  • (Spinach)
  • Chlorophyll-protein
  • Fluorescence
  • Pheophytin
  • Photosystem II
  • Reaction center

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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