Identity of the Photosystem II reaction center polypeptide

H. Y. Nakatani; B. Ke; E. Dolan; C. J. Arntzen

doi:10.1016/0005-2728(84)90175-0

Identity of the Photosystem II reaction center polypeptide

H. Y. Nakatani, B. Ke, E. Dolan, C. J. Arntzen

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47.

Original language	English (US)
Pages (from-to)	347-352
Number of pages	6
Journal	BBA - Bioenergetics
Volume	765
Issue number	3
DOIs	https://doi.org/10.1016/0005-2728(84)90175-0
State	Published - Jun 26 1984
Externally published	Yes

Keywords

(Spinach)
Chlorophyll-protein
Fluorescence
Pheophytin
Photosystem II
Reaction center

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(84)90175-0

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@article{f0ab51322f2b44128131c4279c9fcd69,

title = "Identity of the Photosystem II reaction center polypeptide",

abstract = "A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47.",

keywords = "(Spinach), Chlorophyll-protein, Fluorescence, Pheophytin, Photosystem II, Reaction center",

author = "Nakatani, {H. Y.} and B. Ke and E. Dolan and Arntzen, {C. J.}",

note = "Funding Information: We thank Dr. T.Y. Kuang and Ms. Kathy Li for helpful discussionsa nd assistanceT. his re-searchw as supportedin part by NSF grant No. PCM-8023031a nd DOE Contract No. ACO2-76ERO-1338.",

year = "1984",

month = jun,

day = "26",

doi = "10.1016/0005-2728(84)90175-0",

language = "English (US)",

volume = "765",

pages = "347--352",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Identity of the Photosystem II reaction center polypeptide

AU - Nakatani, H. Y.

AU - Ke, B.

AU - Dolan, E.

AU - Arntzen, C. J.

N1 - Funding Information: We thank Dr. T.Y. Kuang and Ms. Kathy Li for helpful discussionsa nd assistanceT. his re-searchw as supportedin part by NSF grant No. PCM-8023031a nd DOE Contract No. ACO2-76ERO-1338.

PY - 1984/6/26

Y1 - 1984/6/26

N2 - A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47.

AB - A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47.

KW - (Spinach)

KW - Chlorophyll-protein

KW - Fluorescence

KW - Pheophytin

KW - Photosystem II

KW - Reaction center

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U2 - 10.1016/0005-2728(84)90175-0

DO - 10.1016/0005-2728(84)90175-0

M3 - Article

AN - SCOPUS:0001453095

SN - 0005-2728

VL - 765

SP - 347

EP - 352

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

IS - 3

ER -

Identity of the Photosystem II reaction center polypeptide

Abstract

Keywords

ASJC Scopus subject areas

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