Identifying single bases in a DNA oligomer with electron tunnelling

Shuo Huang; Jin He; Shuai Chang; Peiming Zhang; Feng Liang; Shengqin Li; Michael Tuchband; Alexander Fuhrmann; Robert Ros; Stuart Lindsay

doi:10.1038/nnano.2010.213

Identifying single bases in a DNA oligomer with electron tunnelling

Shuo Huang, Jin He, Shuai Chang, Peiming Zhang, Feng Liang, Shengqin Li, Michael Tuchband, Alexander Fuhrmann, Robert Ros, Stuart Lindsay

Research output: Contribution to journal › Article › peer-review

255 Scopus citations

Abstract

It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore^1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing^3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides⁶ and nucleosides ⁷. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.

Original language	English (US)
Pages (from-to)	868-873
Number of pages	6
Journal	Nature nanotechnology
Volume	5
Issue number	12
DOIs	https://doi.org/10.1038/nnano.2010.213
State	Published - Dec 2010

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Biomedical Engineering
General Materials Science
Condensed Matter Physics
Electrical and Electronic Engineering

Access to Document

10.1038/nnano.2010.213

Cite this

@article{7e72e3a2bbfc4791a01c2d6594f22c09,

title = "Identifying single bases in a DNA oligomer with electron tunnelling",

abstract = "It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides6 and nucleosides 7. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.",

author = "Shuo Huang and Jin He and Shuai Chang and Peiming Zhang and Feng Liang and Shengqin Li and Michael Tuchband and Alexander Fuhrmann and Robert Ros and Stuart Lindsay",

note = "Funding Information: The authors acknowledge useful discussions with O. Sankey, P. Krstic and B. Gyarfus. P. Collins made helpful comments on an earlier version of this manuscript. H. Liu composed the graphic for Fig. 1a. This work was supported by a grant from the Sequencing Technology Program of the National Human Genome Research Institute (HG004378). R.R. and A.F. were supported by a grant from the National Cancer Institute (U54CA143682).",

year = "2010",

month = dec,

doi = "10.1038/nnano.2010.213",

language = "English (US)",

volume = "5",

pages = "868--873",

journal = "Nature nanotechnology",

issn = "1748-3387",

publisher = "Nature Publishing Group",

number = "12",

}

TY - JOUR

T1 - Identifying single bases in a DNA oligomer with electron tunnelling

AU - Huang, Shuo

AU - He, Jin

AU - Chang, Shuai

AU - Zhang, Peiming

AU - Liang, Feng

AU - Li, Shengqin

AU - Tuchband, Michael

AU - Fuhrmann, Alexander

AU - Ros, Robert

AU - Lindsay, Stuart

N1 - Funding Information: The authors acknowledge useful discussions with O. Sankey, P. Krstic and B. Gyarfus. P. Collins made helpful comments on an earlier version of this manuscript. H. Liu composed the graphic for Fig. 1a. This work was supported by a grant from the Sequencing Technology Program of the National Human Genome Research Institute (HG004378). R.R. and A.F. were supported by a grant from the National Cancer Institute (U54CA143682).

PY - 2010/12

Y1 - 2010/12

N2 - It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides6 and nucleosides 7. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.

AB - It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides6 and nucleosides 7. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.

UR - http://www.scopus.com/inward/record.url?scp=78650009474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650009474&partnerID=8YFLogxK

U2 - 10.1038/nnano.2010.213

DO - 10.1038/nnano.2010.213

M3 - Article

C2 - 21076404

AN - SCOPUS:78650009474

SN - 1748-3387

VL - 5

SP - 868

EP - 873

JO - Nature nanotechnology

JF - Nature nanotechnology

IS - 12

ER -

Identifying single bases in a DNA oligomer with electron tunnelling

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this