Identifying single bases in a DNA oligomer with electron tunnelling

Shuo Huang, Jin He, Shuai Chang, Peiming Zhang, Feng Liang, Shengqin Li, Michael Tuchband, Alexander Fuhrmann, Robert Ros, Stuart Lindsay

Research output: Contribution to journalArticle

190 Scopus citations

Abstract

It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides6 and nucleosides 7. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.

Original languageEnglish (US)
Pages (from-to)868-873
Number of pages6
JournalNature nanotechnology
Volume5
Issue number12
DOIs
StatePublished - Dec 2010

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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  • Cite this

    Huang, S., He, J., Chang, S., Zhang, P., Liang, F., Li, S., Tuchband, M., Fuhrmann, A., Ros, R., & Lindsay, S. (2010). Identifying single bases in a DNA oligomer with electron tunnelling. Nature nanotechnology, 5(12), 868-873. https://doi.org/10.1038/nnano.2010.213