Identifying single bases in a DNA oligomer with electron tunnelling

Shuo Huang, Jin He, Shuai Chang, Peiming Zhang, Feng Liang, Shengqin Li, Michael Tuchband, Alexander Fuhrmann, Robert Ros, Stuart Lindsay

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

It has been proposed that single molecules of DNA could be sequenced by measuring the physical properties of the bases as they pass through a nanopore1,2. Theoretical calculations suggest that electron tunnelling can identify bases in single-stranded DNA without enzymatic processing3-5, and it was recently experimentally shown that tunnelling can sense individual nucleotides6 and nucleosides 7. Here, we report that tunnelling electrodes functionalized with recognition reagents can identify a single base flanked by other bases in short DNA oligomers. The residence time of a single base in a recognition junction is on the order of a second, but pulling the DNA through the junction with a force of tens of piconewtons would yield reading speeds of tens of bases per second.

Original languageEnglish (US)
Pages (from-to)868-873
Number of pages6
JournalNature Nanotechnology
Volume5
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Electron tunneling
electron tunneling
oligomers
Oligomers
DNA
deoxyribonucleic acid
Single-Stranded DNA
Nucleosides
nucleosides
pulling
Physical properties
reagents
Electrodes
Molecules
physical properties
electrodes
molecules

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics

Cite this

Identifying single bases in a DNA oligomer with electron tunnelling. / Huang, Shuo; He, Jin; Chang, Shuai; Zhang, Peiming; Liang, Feng; Li, Shengqin; Tuchband, Michael; Fuhrmann, Alexander; Ros, Robert; Lindsay, Stuart.

In: Nature Nanotechnology, Vol. 5, No. 12, 12.2010, p. 868-873.

Research output: Contribution to journalArticle

Huang, S, He, J, Chang, S, Zhang, P, Liang, F, Li, S, Tuchband, M, Fuhrmann, A, Ros, R & Lindsay, S 2010, 'Identifying single bases in a DNA oligomer with electron tunnelling', Nature Nanotechnology, vol. 5, no. 12, pp. 868-873. https://doi.org/10.1038/nnano.2010.213
Huang, Shuo ; He, Jin ; Chang, Shuai ; Zhang, Peiming ; Liang, Feng ; Li, Shengqin ; Tuchband, Michael ; Fuhrmann, Alexander ; Ros, Robert ; Lindsay, Stuart. / Identifying single bases in a DNA oligomer with electron tunnelling. In: Nature Nanotechnology. 2010 ; Vol. 5, No. 12. pp. 868-873.
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