Identification of wastewater bacteria involved in the degradation of triclocarban and its non-chlorinated congener

Todd R. Miller, David R. Colquhoun, Rolf Halden

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Triclocarban (TCC) is an antimicrobial additive of personal care products that is only partially degraded during wastewater treatment. Bacteria responsible for its transformation are unknown. We obtained wastewater bacteria capable of using as the sole carbon source TCC or its non-chlorinated analog, carbanilide (NCC). Enrichments established using activated sludge amended with TCC and NCC, respectively, were maintained for 1 year through successive transfers. Enrichments displayed exponential growth after 2 weeks, reaching stationary phase after 1 month. The NCC enrichment was shown to accumulate aniline. Denaturing gradient gel electrophoresis of amplified 16S rRNA genes indicated markedly reduced community richness compared to the inoculum and a single, prominent taxonomic unit emerged in both chlorinated and non-chlorinated carbanilide enrichment cultures. Cloned 16S rRNA genes showed both enrichments were dominated by a single genotype related to uncharacterized organisms within the Alcaligenaceae. Of ~30 sequences from each enrichment, no other organisms were detected in the TCC enrichment while, a small, flanking community of alpha proteobacteria was detected in the NCC enrichment. Study results demonstrate that growth of wastewater bacteria on TCC and its lower chlorinated analog can be linked to bacteria within the family Alcaligenaceae. These organisms are promising agents for the bioremediation of hazardous phenylurea pollutants.

Original languageEnglish (US)
Pages (from-to)766-772
Number of pages7
JournalJournal of Hazardous Materials
Volume183
Issue number1-3
DOIs
StatePublished - Nov 2010

Fingerprint

Carbanilides
Waste Water
Bacteria
Wastewater
Alcaligenaceae
wastewater
Degradation
degradation
bacterium
rRNA Genes
Genes
Bioremediation
Alphaproteobacteria
Aniline
Electrophoresis
Denaturing Gradient Gel Electrophoresis
Cell culture
Environmental Biodegradation
Wastewater treatment
Growth

Keywords

  • Alcaligenes
  • Antimicrobial
  • Carbanilide
  • Phenylurea herbicide
  • Triclocarban
  • Wastewater bacteria

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Identification of wastewater bacteria involved in the degradation of triclocarban and its non-chlorinated congener. / Miller, Todd R.; Colquhoun, David R.; Halden, Rolf.

In: Journal of Hazardous Materials, Vol. 183, No. 1-3, 11.2010, p. 766-772.

Research output: Contribution to journalArticle

@article{3e5cb396713f4457a8b7f7a8c127bf31,
title = "Identification of wastewater bacteria involved in the degradation of triclocarban and its non-chlorinated congener",
abstract = "Triclocarban (TCC) is an antimicrobial additive of personal care products that is only partially degraded during wastewater treatment. Bacteria responsible for its transformation are unknown. We obtained wastewater bacteria capable of using as the sole carbon source TCC or its non-chlorinated analog, carbanilide (NCC). Enrichments established using activated sludge amended with TCC and NCC, respectively, were maintained for 1 year through successive transfers. Enrichments displayed exponential growth after 2 weeks, reaching stationary phase after 1 month. The NCC enrichment was shown to accumulate aniline. Denaturing gradient gel electrophoresis of amplified 16S rRNA genes indicated markedly reduced community richness compared to the inoculum and a single, prominent taxonomic unit emerged in both chlorinated and non-chlorinated carbanilide enrichment cultures. Cloned 16S rRNA genes showed both enrichments were dominated by a single genotype related to uncharacterized organisms within the Alcaligenaceae. Of ~30 sequences from each enrichment, no other organisms were detected in the TCC enrichment while, a small, flanking community of alpha proteobacteria was detected in the NCC enrichment. Study results demonstrate that growth of wastewater bacteria on TCC and its lower chlorinated analog can be linked to bacteria within the family Alcaligenaceae. These organisms are promising agents for the bioremediation of hazardous phenylurea pollutants.",
keywords = "Alcaligenes, Antimicrobial, Carbanilide, Phenylurea herbicide, Triclocarban, Wastewater bacteria",
author = "Miller, {Todd R.} and Colquhoun, {David R.} and Rolf Halden",
year = "2010",
month = "11",
doi = "10.1016/j.jhazmat.2010.07.092",
language = "English (US)",
volume = "183",
pages = "766--772",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Identification of wastewater bacteria involved in the degradation of triclocarban and its non-chlorinated congener

AU - Miller, Todd R.

AU - Colquhoun, David R.

AU - Halden, Rolf

PY - 2010/11

Y1 - 2010/11

N2 - Triclocarban (TCC) is an antimicrobial additive of personal care products that is only partially degraded during wastewater treatment. Bacteria responsible for its transformation are unknown. We obtained wastewater bacteria capable of using as the sole carbon source TCC or its non-chlorinated analog, carbanilide (NCC). Enrichments established using activated sludge amended with TCC and NCC, respectively, were maintained for 1 year through successive transfers. Enrichments displayed exponential growth after 2 weeks, reaching stationary phase after 1 month. The NCC enrichment was shown to accumulate aniline. Denaturing gradient gel electrophoresis of amplified 16S rRNA genes indicated markedly reduced community richness compared to the inoculum and a single, prominent taxonomic unit emerged in both chlorinated and non-chlorinated carbanilide enrichment cultures. Cloned 16S rRNA genes showed both enrichments were dominated by a single genotype related to uncharacterized organisms within the Alcaligenaceae. Of ~30 sequences from each enrichment, no other organisms were detected in the TCC enrichment while, a small, flanking community of alpha proteobacteria was detected in the NCC enrichment. Study results demonstrate that growth of wastewater bacteria on TCC and its lower chlorinated analog can be linked to bacteria within the family Alcaligenaceae. These organisms are promising agents for the bioremediation of hazardous phenylurea pollutants.

AB - Triclocarban (TCC) is an antimicrobial additive of personal care products that is only partially degraded during wastewater treatment. Bacteria responsible for its transformation are unknown. We obtained wastewater bacteria capable of using as the sole carbon source TCC or its non-chlorinated analog, carbanilide (NCC). Enrichments established using activated sludge amended with TCC and NCC, respectively, were maintained for 1 year through successive transfers. Enrichments displayed exponential growth after 2 weeks, reaching stationary phase after 1 month. The NCC enrichment was shown to accumulate aniline. Denaturing gradient gel electrophoresis of amplified 16S rRNA genes indicated markedly reduced community richness compared to the inoculum and a single, prominent taxonomic unit emerged in both chlorinated and non-chlorinated carbanilide enrichment cultures. Cloned 16S rRNA genes showed both enrichments were dominated by a single genotype related to uncharacterized organisms within the Alcaligenaceae. Of ~30 sequences from each enrichment, no other organisms were detected in the TCC enrichment while, a small, flanking community of alpha proteobacteria was detected in the NCC enrichment. Study results demonstrate that growth of wastewater bacteria on TCC and its lower chlorinated analog can be linked to bacteria within the family Alcaligenaceae. These organisms are promising agents for the bioremediation of hazardous phenylurea pollutants.

KW - Alcaligenes

KW - Antimicrobial

KW - Carbanilide

KW - Phenylurea herbicide

KW - Triclocarban

KW - Wastewater bacteria

UR - http://www.scopus.com/inward/record.url?scp=77956434302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956434302&partnerID=8YFLogxK

U2 - 10.1016/j.jhazmat.2010.07.092

DO - 10.1016/j.jhazmat.2010.07.092

M3 - Article

C2 - 20727675

AN - SCOPUS:77956434302

VL - 183

SP - 766

EP - 772

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

IS - 1-3

ER -