Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution.

Layla Parker-Katiraee, Andrew R. Carson, Takahiro Yamada, Philippe Arnaud, Robert Feil, Sayeda N. Abu-Amero, Gudrun E. Moore, Masahiro Kaneda, George H. Perry, Anne Stone, Charles Lee, Makiko Meguro-Horike, Hiroyuki Sasaki, Keiko Kobayashi, Kazuhiko Nakabayashi, Stephen W. Scherer

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Abstract

Imprinted genes are expressed in a parent-of-origin manner and are located in clusters throughout the genome. Aberrations in the expression of imprinted genes on human Chromosome 7 have been suggested to play a role in the etiologies of Russell-Silver Syndrome and autism. We describe the imprinting of KLF14, an intronless member of the Krüppel-like family of transcription factors located at Chromosome 7q32. We show that it has monoallelic maternal expression in all embryonic and extra-embryonic tissues studied, in both human and mouse. We examine epigenetic modifications in the KLF14 CpG island in both species and find this region to be hypomethylated. In addition, we perform chromatin immunoprecipitation and find that the murine Klf14 CpG island lacks allele-specific histone modifications. Despite the absence of these defining features, our analysis of Klf14 in offspring from DNA methyltransferase 3a conditional knockout mice reveals that the gene's expression is dependent upon a maternally methylated region. Due to the intronless nature of Klf14 and its homology to Klf16, we suggest that the gene is an ancient retrotransposed copy of Klf16. By sequence analysis of numerous species, we place the timing of this event after the divergence of Marsupialia, yet prior to the divergence of the Xenarthra superclade. We identify a large number of sequence variants in KLF14 and, using several measures of diversity, we determine that there is greater variability in the human lineage with a significantly increased number of nonsynonymous changes, suggesting human-specific accelerated evolution. Thus, KLF14 may be the first example of an imprinted transcript undergoing accelerated evolution in the human lineage.

Original languageEnglish (US)
JournalPLoS Genetics
Volume3
Issue number5
DOIs
StatePublished - May 4 2007

Fingerprint

Transcription Factors
transcription factors
CpG Islands
gene
chromosome
divergence
imprinting
Xenarthra
Silver-Russell Syndrome
Histone Code
etiology
mice
homology
Gene Expression
Marsupialia
Chromosomes, Human, Pair 7
gene expression
Chromatin Immunoprecipitation
silver
allele

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Parker-Katiraee, L., Carson, A. R., Yamada, T., Arnaud, P., Feil, R., Abu-Amero, S. N., ... Scherer, S. W. (2007). Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution. PLoS Genetics, 3(5). https://doi.org/10.1371/journal.pgen.0030065

Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution. / Parker-Katiraee, Layla; Carson, Andrew R.; Yamada, Takahiro; Arnaud, Philippe; Feil, Robert; Abu-Amero, Sayeda N.; Moore, Gudrun E.; Kaneda, Masahiro; Perry, George H.; Stone, Anne; Lee, Charles; Meguro-Horike, Makiko; Sasaki, Hiroyuki; Kobayashi, Keiko; Nakabayashi, Kazuhiko; Scherer, Stephen W.

In: PLoS Genetics, Vol. 3, No. 5, 04.05.2007.

Research output: Contribution to journalArticle

Parker-Katiraee, L, Carson, AR, Yamada, T, Arnaud, P, Feil, R, Abu-Amero, SN, Moore, GE, Kaneda, M, Perry, GH, Stone, A, Lee, C, Meguro-Horike, M, Sasaki, H, Kobayashi, K, Nakabayashi, K & Scherer, SW 2007, 'Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution.', PLoS Genetics, vol. 3, no. 5. https://doi.org/10.1371/journal.pgen.0030065
Parker-Katiraee, Layla ; Carson, Andrew R. ; Yamada, Takahiro ; Arnaud, Philippe ; Feil, Robert ; Abu-Amero, Sayeda N. ; Moore, Gudrun E. ; Kaneda, Masahiro ; Perry, George H. ; Stone, Anne ; Lee, Charles ; Meguro-Horike, Makiko ; Sasaki, Hiroyuki ; Kobayashi, Keiko ; Nakabayashi, Kazuhiko ; Scherer, Stephen W. / Identification of the imprinted KLF14 transcription factor undergoing human-specific accelerated evolution. In: PLoS Genetics. 2007 ; Vol. 3, No. 5.
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