Identification of S2 as the sensitive state to alkaline photoinactivation of Photosystem II in chloroplasts

J. M. Briantais, C. Vernotte, J. Lavergne, C. J. Arntzen

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

1. Chloroplasts have been preilluminated by a sequence of n short saturating flashes immediately before alkalinization to pH 9.3, and brought back 2 min later to pH 7.8. The assay of Photosystem II activity through dichlorophenolindophenol photoreduction, oxygen evolution, fluorescence induction, shows that part of the centers is inactivated and that this part depends on the number of preilluminating flashes (maximum inhibition after one flash) in a way which suggests identification of state S2 as the target for alkaline inactivation. 2. As shown by Reimer and Trebst ((1975) Biochem. Physiol. Pflanz. 168, 225-232) the inactivation necessitates the presence of gramicidin, which shows that the sensitive site is on the internal side of the thylakoid membrane. 3. The electron flow through inactivated Photosystem II is restored by artificial donor addition (diphenylcarbazide or hydroxylamine); this suggests that the water-splitting enzyme itself is blocked. The inactivation is accompanied by a solubilization of bound Mn2+ and by the occurrence of EPR Signal II "fast". 4. Glutaraldehyde fixation before the treatment does not prevent the inactivation which thus does not seem to involve a protein structural change.

Original languageEnglish (US)
Pages (from-to)61-74
Number of pages14
JournalBBA - Bioenergetics
Volume461
Issue number1
DOIs
StatePublished - Jul 7 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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