Identification of ribonuclease P activity from chick embryos

Emma J. Bowman, Sidney Altman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

RNAase P (EC 3.1.26.5) activity has been identified in chick embryo thigh tissue on the basis of specific cleavage of Escherichia coli 129 nucleotide tRNATyr precursor and has been partially purified by the procedure used for human tissue culture KB cell RNAase P. RNAase P from chick resembles the KB cell RNAase P in substrate specificity, requirement for a divalent cation (Mg2+) and a monovalent cation (K+, Na+ or NH4 +) for activity, inhibition by bulk tRNA, ready inactivation by proteases, and increasing instability with purification. RNAase P activity is also present in whole chick embryos, as well as in liver and heart tissues. Furthermore, crude preparations of RNAase P from chick embryo heart tissue are relatively free of contaminating nucleases.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalBBA - Enzymology
Volume613
Issue number2
DOIs
StatePublished - Jun 13 1980
Externally publishedYes

Fingerprint

Ribonuclease P
Chick Embryo
KB Cells
RNA, Transfer, Tyr
Monovalent Cations
Divalent Cations
Substrate Specificity
Transfer RNA
Thigh
Peptide Hydrolases
Nucleotides
Escherichia coli
Liver

Keywords

  • (Chick embryo)
  • RNAse P
  • tRNA precursor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Identification of ribonuclease P activity from chick embryos. / Bowman, Emma J.; Altman, Sidney.

In: BBA - Enzymology, Vol. 613, No. 2, 13.06.1980, p. 439-447.

Research output: Contribution to journalArticle

Bowman, Emma J. ; Altman, Sidney. / Identification of ribonuclease P activity from chick embryos. In: BBA - Enzymology. 1980 ; Vol. 613, No. 2. pp. 439-447.
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