Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Erica M. Hartmann, David R. Colquhoun, Rolf Halden

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

Original languageEnglish (US)
Pages (from-to)1470-1472
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number7
DOIs
StatePublished - Aug 9 2010

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Keywords

  • Bioremediation
  • Matrix-assisted laser desorption/ionization (MALDI)
  • Proteomics
  • Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE)
  • Toluene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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