Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Erica M. Hartmann, David R. Colquhoun, Rolf Halden

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

Original languageEnglish (US)
Pages (from-to)1470-1472
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number7
DOIs
StatePublished - 2010

Fingerprint

Burkholderia
Peptide Mapping
Toluene
Biomarkers
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Peptides
Pseudomonas putida
Bacteria
Degradation
Enzymes

Keywords

  • Bioremediation
  • Matrix-assisted laser desorption/ionization (MALDI)
  • Proteomics
  • Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE)
  • Toluene

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

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abstract = "The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.",
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AU - Colquhoun, David R.

AU - Halden, Rolf

PY - 2010

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AB - The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

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KW - Proteomics

KW - Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE)

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