Identification of precursor molecules to individual tRNA species from Bombyx mori

R. L. Garber, M. A.Q. Siddiqui, S. Altman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Short labeled 4.5S RNA molecules isolated from the posterior silk gland of Bombyx mori can be separated by two dimensional polyacrylamide gel electrophoresis into many discrete species, some of which are radiochemically pure by the criteria of RNA fingerprinting. One region of the gel contains two precursor RNAs, one to each of the known alanine transfer RNAs. Each precursor tRNA(Ala) molecule contains all of the internal oligonucleotides present in the corresponding tRNA(Ala) species plus new 5' and 3' terminal sequences. Precursor molecules to tRNA1(Gly) (which differ from each other in size) are contained in two other gel regions, and a fourth region contains a precursor to tRNA2(Gly). Both of the transcription initiator purine tetraphosphate nucleosides are present in unfractionated tRNA precursor mixtures, with pppA- predominating over pppG-. Minor nucleotides are also present in B. mori tRNA precursors. No polycistronic tRNA gene transcripts were observed.

Original languageEnglish (US)
Pages (from-to)635-639
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number2
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

ASJC Scopus subject areas

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