Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis

Yameng Liu, Alan Jiang, Estelle Kim, Clarissa Ro, Tayloria Adams, Lisa A. Flanagan, Thomas Taylor, Mark Hayes

Research output: Contribution to journalArticle

Abstract

Neural stem and progenitor cells (NSPCs) are an extremely important group of cells that form the central nervous system during development and have the potential to repair damage in conditions such as stroke impairment, spinal cord injury and Parkinson's disease degradation. Current schemes for separation of NSPCs are inadequate due to the complexity and diversity of cells in the population and lack sufficient markers to distinguish diverse cell types. This study presents an unbiased high-resolution separation and characterization of NSPC subpopulations using direct current insulator-based dielectrophoresis (DC-iDEP). The properties of the cells were identified by the ratio of electrokinetic (EK) to dielectrophoretic (DEP) mobilities. The ratio factor of NSPCs showed more heterogeneity variance (SD = 3.4-3.9) than the controlled more homogeneous human embryonic kidney cells (SD = 1.1), supporting the presence of distinct subpopulations of cells in NSPC cultures. This measure reflected NSPC fate potential since the ratio factor distribution of more neurogenic populations of NSPCs was distinct from the distribution of astrogenic NSPC populations (confidence level >99.9%). The abundance of NSPCs captured with different ranges of ratio of EK to DEP mobilities also exhibit final fate trends consistent with established final fates of the chosen samples. DC-iDEP is a novel, label-free and non-destructive method for differentiating and characterizing, and potentially separating, neural stem cell subpopulations that differ in fate.

Original languageEnglish (US)
Pages (from-to)4066-4072
Number of pages7
JournalAnalyst
Volume144
Issue number13
DOIs
StatePublished - Jul 7 2019

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Neural Stem Cells
Electrophoresis
subpopulation
Stem Cells
Cells
stem
Neurology
Stem cells
Cell culture
Labels
Repair
Degradation
Population
nervous system
Spinal Cord Diseases
repair
Spinal Cord Injuries
Parkinson Disease
Central Nervous System
Cell Culture Techniques

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis. / Liu, Yameng; Jiang, Alan; Kim, Estelle; Ro, Clarissa; Adams, Tayloria; Flanagan, Lisa A.; Taylor, Thomas; Hayes, Mark.

In: Analyst, Vol. 144, No. 13, 07.07.2019, p. 4066-4072.

Research output: Contribution to journalArticle

Liu, Yameng ; Jiang, Alan ; Kim, Estelle ; Ro, Clarissa ; Adams, Tayloria ; Flanagan, Lisa A. ; Taylor, Thomas ; Hayes, Mark. / Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis. In: Analyst. 2019 ; Vol. 144, No. 13. pp. 4066-4072.
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