Identification of MW polyomavirus, a novel polyomavirus in human stool

Erica A. Siebrasse, Alejandro Reyes, Efrem Lim, Guoyan Zhao, Rajhab S. Mkakosya, Mark J. Manary, Jeffrey I. Gordon, David Wang

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

We have discovered a novel polyomavirus present in multiple human stool samples. The virus was initially identified by shotgun pyrosequencing of DNA purified from virus-like particles isolated from a stool sample collected from a healthy child from Malawi. We subsequently sequenced the virus' 4,927-bp genome, which has been provisionally namedMWpolyomavirus (MWPyV). The virus has genomic features characteristic of the family Polyomaviridae but is highly divergent from other members of this family. It is predicted to encode the large T antigen and small T antigen early proteins and the VP1, VP2, and VP3 structural proteins. A real-time PCR assay was designed and used to screen 514 stool samples from children with diarrhea in St. Louis, MO; 12 specimens were positive for MWPyV. Comparison of the whole-genome sequences of the index Malawi case and one St. Louis case demonstrated that the two strains of MWPyV varied by 5.3% at the nucleotide level. The number of polyomaviruses found in the human body continues to grow, raising the question of how many more species have yet to be identified and what roles they play in humans with and without manifest disease.

Original languageEnglish (US)
Pages (from-to)10321-10326
Number of pages6
JournalJournal of Virology
Volume86
Issue number19
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Fingerprint

Polyomavirus
Polyomaviridae
Malawi
Viral Tumor Antigens
Viruses
viruses
Genome
antigens
DNA Viruses
virus-like particles
genome
structural proteins
Firearms
Human Body
sampling
Virion
Real-Time Polymerase Chain Reaction
Diarrhea
diarrhea
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Siebrasse, E. A., Reyes, A., Lim, E., Zhao, G., Mkakosya, R. S., Manary, M. J., ... Wang, D. (2012). Identification of MW polyomavirus, a novel polyomavirus in human stool. Journal of Virology, 86(19), 10321-10326. https://doi.org/10.1128/JVI.01210-12

Identification of MW polyomavirus, a novel polyomavirus in human stool. / Siebrasse, Erica A.; Reyes, Alejandro; Lim, Efrem; Zhao, Guoyan; Mkakosya, Rajhab S.; Manary, Mark J.; Gordon, Jeffrey I.; Wang, David.

In: Journal of Virology, Vol. 86, No. 19, 01.10.2012, p. 10321-10326.

Research output: Contribution to journalArticle

Siebrasse, EA, Reyes, A, Lim, E, Zhao, G, Mkakosya, RS, Manary, MJ, Gordon, JI & Wang, D 2012, 'Identification of MW polyomavirus, a novel polyomavirus in human stool', Journal of Virology, vol. 86, no. 19, pp. 10321-10326. https://doi.org/10.1128/JVI.01210-12
Siebrasse EA, Reyes A, Lim E, Zhao G, Mkakosya RS, Manary MJ et al. Identification of MW polyomavirus, a novel polyomavirus in human stool. Journal of Virology. 2012 Oct 1;86(19):10321-10326. https://doi.org/10.1128/JVI.01210-12
Siebrasse, Erica A. ; Reyes, Alejandro ; Lim, Efrem ; Zhao, Guoyan ; Mkakosya, Rajhab S. ; Manary, Mark J. ; Gordon, Jeffrey I. ; Wang, David. / Identification of MW polyomavirus, a novel polyomavirus in human stool. In: Journal of Virology. 2012 ; Vol. 86, No. 19. pp. 10321-10326.
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