Identification of learning-induced changes in protein networks in the hippocampi of a mouse model of Alzheimer's disease

E. Ferreira, D. M. Shaw, Salvatore Oddo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Memory loss is the most profound clinical manifestation in Alzheimer's disease (AD); however, the molecular mechanisms underlying these deficits are poorly understood. Identification of the molecular pathways involved in the onset of cognitive deficits may lead to the identification of key events in the pathogenesis of AD. Using isobaric tags for relative and absolute quantitation (iTRAQ) and proteomic methods, here we identified learning-induced changes in the hippocampal proteome of non-transgenic (NonTg) and 3 × Tg-AD mice, a widely used animal model of AD. We found that expression of 192 proteins was differentially regulated by learning in NonTg mice. Notably, of these 192 proteins, only 28 were also differentially regulated by learning in 3 × Tg-AD mice, whereas the levels of 164 proteins were uniquely changed in NonTg mice but not in 3 × Tg-AD mice. These data suggest that during learning, 3 × Tg-AD mice fail to differentially regulate 164 proteins. Gene ontology and protein interaction analyses indicated that these proteins were overrepresented in RNA processing, specifically RNA transport, splicing and mRNA translation initiation pathways. These findings suggest that mRNA-processing events that take place during learning and memory are significantly altered in 3 × Tg-AD mice.

Original languageEnglish (US)
Article numbere849
JournalTranslational Psychiatry
Volume6
Issue number7
DOIs
StatePublished - Jul 5 2016

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Hippocampus
Alzheimer Disease
Learning
Proteins
RNA Transport
RNA Splicing
Gene Ontology
Memory Disorders
Protein Biosynthesis
Proteome
Proteomics
Animal Models
Alzheimer disease, familial, type 3
RNA
Messenger RNA

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry
  • Cellular and Molecular Neuroscience

Cite this

Identification of learning-induced changes in protein networks in the hippocampi of a mouse model of Alzheimer's disease. / Ferreira, E.; Shaw, D. M.; Oddo, Salvatore.

In: Translational Psychiatry, Vol. 6, No. 7, e849, 05.07.2016.

Research output: Contribution to journalArticle

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