Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

Mark R. Smith; Matthew G. Boenzli; Vihangi Hindagolla; Jun Ding; John M. Miller; James E. Hutchison; Jeffrey A. Greenwood; Hagai Abeliovich; Alan T. Bakalinsky

doi:10.1128/AEM.01737-12

Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

Mark R. Smith, Matthew G. Boenzli, Vihangi Hindagolla, Jun Ding, John M. Miller, James E. Hutchison, Jeffrey A. Greenwood, Hagai Abeliovich, Alan T. Bakalinsky

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Positively charged gold nanoparticles (0.8-nm core diameter) reduced yeast survival, but not growth, at a concentration of 10 to 100 μg/ml. Among 17 resistant deletion mutants isolated in a genome-wide screen, highly significant enrichment was observed for respiration-deficient mutants lacking genes encoding proteins associated with the mitochondrion.

Original language	English (US)
Pages (from-to)	728-733
Number of pages	6
Journal	Applied and environmental microbiology
Volume	79
Issue number	2
DOIs	https://doi.org/10.1128/AEM.01737-12
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.01737-12

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Smith, M. R., Boenzli, M. G., Hindagolla, V., Ding, J., Miller, J. M., Hutchison, J. E., Greenwood, J. A., Abeliovich, H., & Bakalinsky, A. T. (2013). Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity. Applied and environmental microbiology, 79(2), 728-733. https://doi.org/10.1128/AEM.01737-12

Smith, MR, Boenzli, MG, Hindagolla, V, Ding, J, Miller, JM, Hutchison, JE, Greenwood, JA, Abeliovich, H & Bakalinsky, AT 2013, 'Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity', Applied and environmental microbiology, vol. 79, no. 2, pp. 728-733. https://doi.org/10.1128/AEM.01737-12

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Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

Abstract

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