Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy

A. M. Jeffrey, T. W. Jing, J. A. DeRose, A. Vaught, D. Rekesh, F. X. Lu, Stuart Lindsay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images alone in a blinded trial. The samples were single-stranded and double-stranded DNA with and without covalent modification by the anti-tumor drug cisplatin. The cisplatin adducts were distinguished by substantial kinking at the drug binding site. The oligomers were 20 bases in length, which was too short to permit the kinking angle to be determined with precision. However, models with a 45° kink gave a better fit to the images of the duplex adducts than models with a 90° kink. A variety of structures was observed for the single-stranded adducts.

Original languageEnglish (US)
Pages (from-to)5896-5900
Number of pages5
JournalNucleic Acids Research
Volume21
Issue number25
StatePublished - Dec 25 1993

Fingerprint

Scanning Tunnelling Microscopy
Cisplatin
Scanning tunneling microscopy
Microscopy
Scanning
DNA
Kink
Drugs
Binding sites
Oligomers
Pharmaceutical Preparations
Artifacts
Tumors
Binding Sites
Tumor
Substrate
Substrates
Angle
cisplatin-DNA adduct
DNA Adducts

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Jeffrey, A. M., Jing, T. W., DeRose, J. A., Vaught, A., Rekesh, D., Lu, F. X., & Lindsay, S. (1993). Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy. Nucleic Acids Research, 21(25), 5896-5900.

Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy. / Jeffrey, A. M.; Jing, T. W.; DeRose, J. A.; Vaught, A.; Rekesh, D.; Lu, F. X.; Lindsay, Stuart.

In: Nucleic Acids Research, Vol. 21, No. 25, 25.12.1993, p. 5896-5900.

Research output: Contribution to journalArticle

Jeffrey, AM, Jing, TW, DeRose, JA, Vaught, A, Rekesh, D, Lu, FX & Lindsay, S 1993, 'Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy', Nucleic Acids Research, vol. 21, no. 25, pp. 5896-5900.
Jeffrey AM, Jing TW, DeRose JA, Vaught A, Rekesh D, Lu FX et al. Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy. Nucleic Acids Research. 1993 Dec 25;21(25):5896-5900.
Jeffrey, A. M. ; Jing, T. W. ; DeRose, J. A. ; Vaught, A. ; Rekesh, D. ; Lu, F. X. ; Lindsay, Stuart. / Identification of DNA - Cisplatin adducts in a blind trial of in situ scanning tunneling microscopy. In: Nucleic Acids Research. 1993 ; Vol. 21, No. 25. pp. 5896-5900.
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