Identification of DNA-cisplatin adducts in a blind trial of in situ scanning tunneling microscopy

A. M. Jeffrey, T. W. Jing, J. A. DeRose, A. Vaught, D. Rekesh, F. X. Lu, Stuart Lindsay

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images alone in a blinded trial. The samples were single-stranded and double-stranded DNA with and without covalent modification by the anti-tumor drug cisplatin. The cisplatin adducts were distinguished by substantial kinking at the drug binding site. The oligomers were 20 bases in length, which was too short to permit the kinking angle to be determined with precision. However, models with a 45° kink gave a better fit to the images of the duplex adducts than models with a 90° kink. A variety of structures was observed for the single-stranded adducts.

Original languageEnglish (US)
Pages (from-to)5896-5900
Number of pages5
JournalNucleic acids research
Volume21
Issue number25
DOIs
StatePublished - Dec 25 1993

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Identification of DNA-cisplatin adducts in a blind trial of in situ scanning tunneling microscopy'. Together they form a unique fingerprint.

  • Cite this