Abstract
The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1363-1365 |
| Number of pages | 3 |
| Journal | Review of Scientific Instruments |
| Volume | 75 |
| Issue number | 5 PART 1 |
| DOIs | |
| State | Published - May 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Instrumentation
- Physics and Astronomy (miscellaneous)
Cite this
Identification of budding yeast using a fiber-optic imaging bundle. / Koschwanez, John; Holl, Mark; Marquardt, Brian; Dragavon, Joe; Burgess, Lloyd; Meldrum, Deirdre.
In: Review of Scientific Instruments, Vol. 75, No. 5 PART 1, 05.2004, p. 1363-1365.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of budding yeast using a fiber-optic imaging bundle
AU - Koschwanez, John
AU - Holl, Mark
AU - Marquardt, Brian
AU - Dragavon, Joe
AU - Burgess, Lloyd
AU - Meldrum, Deirdre
PY - 2004/5
Y1 - 2004/5
N2 - The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.
AB - The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.
UR - http://www.scopus.com/inward/record.url?scp=3042693134&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042693134&partnerID=8YFLogxK
U2 - 10.1063/1.1710702
DO - 10.1063/1.1710702
M3 - Article
AN - SCOPUS:3042693134
VL - 75
SP - 1363
EP - 1365
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
SN - 0034-6748
IS - 5 PART 1
ER -