Identification of budding yeast using a fiber-optic imaging bundle

John Koschwanez, Mark Holl, Brian Marquardt, Joe Dragavon, Lloyd Burgess, Deirdre Meldrum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

Original languageEnglish (US)
Pages (from-to)1363-1365
Number of pages3
JournalReview of Scientific Instruments
Volume75
Issue number5 PART 1
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

yeast
Yeast
Fiber optics
bundles
fiber optics
Imaging techniques
cells
Cells
Microfluidics
Image analysis
image analysis

ASJC Scopus subject areas

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Cite this

Koschwanez, J., Holl, M., Marquardt, B., Dragavon, J., Burgess, L., & Meldrum, D. (2004). Identification of budding yeast using a fiber-optic imaging bundle. Review of Scientific Instruments, 75(5 PART 1), 1363-1365. https://doi.org/10.1063/1.1710702

Identification of budding yeast using a fiber-optic imaging bundle. / Koschwanez, John; Holl, Mark; Marquardt, Brian; Dragavon, Joe; Burgess, Lloyd; Meldrum, Deirdre.

In: Review of Scientific Instruments, Vol. 75, No. 5 PART 1, 05.2004, p. 1363-1365.

Research output: Contribution to journalArticle

Koschwanez, J, Holl, M, Marquardt, B, Dragavon, J, Burgess, L & Meldrum, D 2004, 'Identification of budding yeast using a fiber-optic imaging bundle', Review of Scientific Instruments, vol. 75, no. 5 PART 1, pp. 1363-1365. https://doi.org/10.1063/1.1710702
Koschwanez J, Holl M, Marquardt B, Dragavon J, Burgess L, Meldrum D. Identification of budding yeast using a fiber-optic imaging bundle. Review of Scientific Instruments. 2004 May;75(5 PART 1):1363-1365. https://doi.org/10.1063/1.1710702
Koschwanez, John ; Holl, Mark ; Marquardt, Brian ; Dragavon, Joe ; Burgess, Lloyd ; Meldrum, Deirdre. / Identification of budding yeast using a fiber-optic imaging bundle. In: Review of Scientific Instruments. 2004 ; Vol. 75, No. 5 PART 1. pp. 1363-1365.
@article{70e63651dde7427a998545347d4a468f,
title = "Identification of budding yeast using a fiber-optic imaging bundle",
abstract = "The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.",
author = "John Koschwanez and Mark Holl and Brian Marquardt and Joe Dragavon and Lloyd Burgess and Deirdre Meldrum",
year = "2004",
month = "5",
doi = "10.1063/1.1710702",
language = "English (US)",
volume = "75",
pages = "1363--1365",
journal = "Review of Scientific Instruments",
issn = "0034-6748",
publisher = "American Institute of Physics Publising LLC",
number = "5 PART 1",

}

TY - JOUR

T1 - Identification of budding yeast using a fiber-optic imaging bundle

AU - Koschwanez, John

AU - Holl, Mark

AU - Marquardt, Brian

AU - Dragavon, Joe

AU - Burgess, Lloyd

AU - Meldrum, Deirdre

PY - 2004/5

Y1 - 2004/5

N2 - The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

AB - The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

UR - http://www.scopus.com/inward/record.url?scp=3042693134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042693134&partnerID=8YFLogxK

U2 - 10.1063/1.1710702

DO - 10.1063/1.1710702

M3 - Article

AN - SCOPUS:3042693134

VL - 75

SP - 1363

EP - 1365

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

SN - 0034-6748

IS - 5 PART 1

ER -