Identification of budding yeast using a fiber-optic imaging bundle

John Koschwanez; Mark Holl; Brian Marquardt; Joe Dragavon; Lloyd Burgess; Deirdre Meldrum

doi:10.1063/1.1710702

Identification of budding yeast using a fiber-optic imaging bundle

John Koschwanez, Mark Holl, Brian Marquardt, Joe Dragavon, Lloyd Burgess, Deirdre Meldrum

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

Original language	English (US)
Pages (from-to)	1363-1365
Number of pages	3
Journal	Review of Scientific Instruments
Volume	75
Issue number	5 PART 1
DOIs	https://doi.org/10.1063/1.1710702
State	Published - May 2004
Externally published	Yes

ASJC Scopus subject areas

Instrumentation

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author = "John Koschwanez and Mark Holl and Brian Marquardt and Joe Dragavon and Lloyd Burgess and Deirdre Meldrum",

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AU - Holl, Mark

AU - Marquardt, Brian

AU - Dragavon, Joe

AU - Burgess, Lloyd

AU - Meldrum, Deirdre

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AB - The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

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SN - 0034-6748

IS - 5 PART 1

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Identification of budding yeast using a fiber-optic imaging bundle

Abstract

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