Identification of budding yeast using a fiber-optic imaging bundle

John Koschwanez, Mark Holl, Brian Marquardt, Joe Dragavon, Lloyd Burgess, Deirdre Meldrum

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The use of fibre-optic imaging bundle to identify single yeast cells was described. The system classified the cell as budding or not budding a daughter cell. It was integrated into a microfluidic system to automate yeast pedigree analysis. The device hold a single yeast cell in place and deliver each bud to a specified location an agar plate. The image analysis was required to detect trapped mother cell, budding mother cell and budding event. The fibre-optic imaging bundles provided dense packing alternative for continuous observation of all budding events in parallel.

Original languageEnglish (US)
Pages (from-to)1363-1365
Number of pages3
JournalReview of Scientific Instruments
Volume75
Issue number5 PART 1
DOIs
StatePublished - May 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Instrumentation

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  • Cite this

    Koschwanez, J., Holl, M., Marquardt, B., Dragavon, J., Burgess, L., & Meldrum, D. (2004). Identification of budding yeast using a fiber-optic imaging bundle. Review of Scientific Instruments, 75(5 PART 1), 1363-1365. https://doi.org/10.1063/1.1710702