Identification of a plant-encoded analog of PKR, the mammalian double-stranded RNA-dependent protein kinase

Jeffrey Langland, Song Jin, Bertram Jacobs, Don A. Roth

Research output: Contribution to journalArticle

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Abstract

Plant virus or viroid infection stimulates the phosphorylation of a plant-encoded protein of Mr 68,000 to 70,000 (now termed pPKR) that is associated with double-stranded RNA-stimulated protein kinase activity. Using various biochemical and immunological comparisons, we have demonstrated that this plant protein is an analog of the mammalian PKR enzymes. pPKR is both cytosolic and ribosome associated, similar to mammalian PKR, and appears to be capable of phosphorylating exogenous histones. Monoclonal antiserum to the human PKR as well as antiserum to a conserved double-stranded RNA-binding domain present on mammalian PKR demonstrated cross-reactivity with pPKR. Likewise, polyclonal antiserum to the pPKR detected the mouse and human PKR in western blot analysis. Northern blot analysis of a mammalian PKR cDNA detected a specific 2.5-kb transcript present in plant poly(A)+ RNA.

Original languageEnglish (US)
Pages (from-to)1259-1267
Number of pages9
JournalPlant Physiology
Volume108
Issue number3
StatePublished - Jul 1995

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eIF-2 Kinase
Double-Stranded RNA
double-stranded RNA
protein kinases
antiserum
Immune Sera
Plant Proteins
Viroids
viroids
Plant Viruses
plant viruses
messenger RNA
plant proteins
ribosomes
Ribosomes
cross reaction
histones
Northern blotting
Northern Blotting
Histones

ASJC Scopus subject areas

  • Plant Science

Cite this

Identification of a plant-encoded analog of PKR, the mammalian double-stranded RNA-dependent protein kinase. / Langland, Jeffrey; Jin, Song; Jacobs, Bertram; Roth, Don A.

In: Plant Physiology, Vol. 108, No. 3, 07.1995, p. 1259-1267.

Research output: Contribution to journalArticle

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