TY - JOUR
T1 - Identification of a new turncurtovirus in the leafhopper Circulifer haematoceps and the host plant species Sesamum indicum
AU - Hasanvand, Vahid
AU - Kamali, Mehdi
AU - Heydarnejad, Jahangir
AU - Massumi, Hossain
AU - Kvarnheden, Anders
AU - Varsani, Arvind
N1 - Funding Information:
Fig. 2 Tanglegram of the maximum likelihood phylogenies of the CP and Rep amino acid sequences of the 61 turncurtoviruses. The tangle-gram shows that the CP and Rep amino acid-derived phylogenies are incongruent thus recombination has likely played a significant role in the evolution of turncurtoviruses. The nucleotide sequences of the rep and cp genes were extracted from 61 genomes and used to generate translated protein sequence datasets for Rep and CP. These sequences Acknowledgements This research work was supported by Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Iran.
Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Turncurtoviruses (family: Geminiviridae; genus: Turncurtovirus) appear to have a high degree of genetic variation in Iran. Leafhoppers of the species Circulifer haematoceps (Mulsant and Rey, 1855) (family: Cicadellidae) were collected in 2014 from three geographical regions in south-eastern Iran (Orzoeyeh, Jiroft and Sirjan; Kerman province) and screened for the presence of turncurtoviruses using a combination of PCR and rolling circle amplification (RCA) methods. Eleven genomes of turncurtovirus were recovered and sequenced. Leafhoppers were sampled off sesame (S. indicum L.) and turnip (Brassica rapa sub sp. rapa). Thus, we identified three symptomatic sesame plants (yellowing, boat-shaped leaf curling, vein swelling on the lower leaf surfaces) from sesame farms in Jiroft. In these samples, we identified the same turncurtovirus as in the leafhoppers and have named it sesame curly top virus (SeCTV). Collectively, these SeCTV share > 98% genome-wide pairwise identity and ~ 87.3% to a recently identified turncurtovirus (sesame yellow mosaic virus; SeYMV) from sesame in Pakistan (GenBank accession MF344550). The SeCTV and SeYMV sequences share < 70% genome-wide pairwise identity with isolates of Turnip curly top virus and Turnip leaf roll virus, the two species in the genus Turncurtovirus. Based on the pairwise identities and phylogenetic analysis, SeCTV (n = 12) and SeYMV (n = 1) represent two strains of a new species in the genus Turncurtovirus.
AB - Turncurtoviruses (family: Geminiviridae; genus: Turncurtovirus) appear to have a high degree of genetic variation in Iran. Leafhoppers of the species Circulifer haematoceps (Mulsant and Rey, 1855) (family: Cicadellidae) were collected in 2014 from three geographical regions in south-eastern Iran (Orzoeyeh, Jiroft and Sirjan; Kerman province) and screened for the presence of turncurtoviruses using a combination of PCR and rolling circle amplification (RCA) methods. Eleven genomes of turncurtovirus were recovered and sequenced. Leafhoppers were sampled off sesame (S. indicum L.) and turnip (Brassica rapa sub sp. rapa). Thus, we identified three symptomatic sesame plants (yellowing, boat-shaped leaf curling, vein swelling on the lower leaf surfaces) from sesame farms in Jiroft. In these samples, we identified the same turncurtovirus as in the leafhoppers and have named it sesame curly top virus (SeCTV). Collectively, these SeCTV share > 98% genome-wide pairwise identity and ~ 87.3% to a recently identified turncurtovirus (sesame yellow mosaic virus; SeYMV) from sesame in Pakistan (GenBank accession MF344550). The SeCTV and SeYMV sequences share < 70% genome-wide pairwise identity with isolates of Turnip curly top virus and Turnip leaf roll virus, the two species in the genus Turncurtovirus. Based on the pairwise identities and phylogenetic analysis, SeCTV (n = 12) and SeYMV (n = 1) represent two strains of a new species in the genus Turncurtovirus.
KW - Circulifer haematoceps
KW - Iran
KW - Sesame curly top virus
KW - Sesamum indicum
KW - Turncurtovirus
UR - http://www.scopus.com/inward/record.url?scp=85055115527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055115527&partnerID=8YFLogxK
U2 - 10.1007/s11262-018-1604-x
DO - 10.1007/s11262-018-1604-x
M3 - Article
C2 - 30311179
AN - SCOPUS:85055115527
SN - 0920-8569
VL - 54
SP - 840
EP - 845
JO - Virus Genes
JF - Virus Genes
IS - 6
ER -