Identification of a 32-34-kilodalton polypeptide as a herbicide receptor protein in Photosystem II

John E. Mullet, Charles J. Arntzen

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Photosystem II particles which retained high rates of herbicide-sensitive activity were used to examine the site(s) of action of various herbicides. A polypeptide of 32-34 kdaltons was identified as the triazine-herbicide binding site based upon: (a) parallel loss of atrazine activity and the polypeptide during either trypsin treatment or selective detergent depletion of protein in the Photosystem II complex, and (b) covalent labeling of the polypeptide by a 14C-labeled photoaffinity triazine. In Photosystem II particles depleted of the 32-34-kdalton polypeptide, electron transport was still active and was slightly sensitive to DCMU and largely sensitive to dinoseb (urea and nitrophenol herbicides, respectively). On the basis of this result it is proposed that the general herbicide binding site common to atrazine, DCMU and dinoseb is formed by a minimum of two polypeptides which determine affinity and/or mediate herbicide-induced inhibition of electron transport on the acceptor side of Photosystem II.

Original languageEnglish (US)
Pages (from-to)236-248
Number of pages13
JournalBBA - Bioenergetics
Volume635
Issue number2
DOIs
StatePublished - Apr 13 1981
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Herbicides
Peptides
Diuron
Atrazine
Triazines
Proteins
Electron Transport
Binding Sites
Nitrophenols
Detergents
Trypsin
Labeling
Urea

Keywords

  • Chloroplast membrane protein
  • Herbicide receptor
  • Photosystem II
  • Triazine herbicide

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Identification of a 32-34-kilodalton polypeptide as a herbicide receptor protein in Photosystem II. / Mullet, John E.; Arntzen, Charles J.

In: BBA - Bioenergetics, Vol. 635, No. 2, 13.04.1981, p. 236-248.

Research output: Contribution to journalArticle

Mullet, John E. ; Arntzen, Charles J. / Identification of a 32-34-kilodalton polypeptide as a herbicide receptor protein in Photosystem II. In: BBA - Bioenergetics. 1981 ; Vol. 635, No. 2. pp. 236-248.
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