Identification and quantitation of lipid C=C location isomers: A shotgun lipidomics approach enabled by photochemical reaction

Xiaoxiao Ma, Leelyn Chong, Ran Tian, Riyi Shi, Ye Hu, Zheng Ouyang, Yu Xia

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The field of lipidomics has been significantly advanced by mass spectrometric analysis. The distinction and quantitation of the unsaturated lipid isomers, however, remain a long-standing challenge. In this study, we have developed an analytical tool for both identification and quantitation of lipid C=C location isomers from complex mixtures using online Paternò-Büchi reaction coupled with tandem mass spectrometry (MS/MS). The potential of this method has been demonstrated with an implementation into shotgun lipid analysis of animal tissues. Among 96 of the unsaturated fatty acids and glycerophospholipids identified from rat brain tissue, 50% of them were found as mixtures of C=C location isomers; for the first time, to our knowledge, the quantitative information of lipid C=C isomers from a broad range of classes was obtained. This method also enabled facile cross-tissue examinations, which revealed significant changes in C=C location isomer compositions of a series of fatty acids and glycerophospholipid (GP) species between the normal and cancerous tissues.

Original languageEnglish (US)
Pages (from-to)2573-2578
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number10
DOIs
StatePublished - Mar 8 2016
Externally publishedYes

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Firearms
Glycerophospholipids
Lipids
Tandem Mass Spectrometry
Complex Mixtures
Unsaturated Fatty Acids
Fatty Acids
Brain

Keywords

  • Cancerous tissue analysis
  • Glycerophospholipids
  • Lipid biomarkers
  • Paternò-Büchi reaction
  • Photochemical reaction

ASJC Scopus subject areas

  • General

Cite this

Identification and quantitation of lipid C=C location isomers : A shotgun lipidomics approach enabled by photochemical reaction. / Ma, Xiaoxiao; Chong, Leelyn; Tian, Ran; Shi, Riyi; Hu, Ye; Ouyang, Zheng; Xia, Yu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 10, 08.03.2016, p. 2573-2578.

Research output: Contribution to journalArticle

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