Identification and molecular characterization of a single-stranded circular DNA virus with similarities to Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1

Zhenguo Du, Yafei Tang, Songbai Zhang, Xiaoman She, Guobing Lan, Arvind Varsani, Zifu He

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A putative circular single-stranded DNA (ssDNA) virus was recovered from Hypericum japonicum collected in Vietnam. The viral isolate was tentatively named Hypericum japonicum-associated circular DNA virus (HJasCV). HJasCV shares 58.7-65.4% nucleotide sequence identity with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) and SsHADV-1-like viruses. Like this group of viruses, the genome of HJasCV (2 200 nt) has two large ORFs, one in the virion-sense and the other in the complementary-sense DNA. The proteins encoded in the virion-sense and complementary-sense ORFs share 39-46 % and 45-67 % amino acid sequence identity with the putative capsid and replication-associated proteins (Reps), respectively, of SsHADV-1 and SsHADV-1-like viruses. The putative Rep of HJasCV contains all of the motifs related to rolling-circle replication. Its 111-bp intergenic region (IR) contains a hairpin structure with a geminivirus-like nonanucleotide sequence, TAATGTTAT, at the apex of the loop. Phylogenetic analysis revealed that HJasCV forms a monophyletic clade with SsHADV-1 and SsHADV-1-like viruses.

Original languageEnglish (US)
Pages (from-to)1527-1531
Number of pages5
JournalArchives of Virology
Volume159
Issue number6
DOIs
StatePublished - 2014
Externally publishedYes

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Satellite Viruses
Circular DNA
Ascomycota
DNA Viruses
Single-Stranded DNA
Hypericum
Viruses
Virion
Open Reading Frames
Geminiviridae
Intergenic DNA
Vietnam
Capsid
Amino Acid Sequence
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

Identification and molecular characterization of a single-stranded circular DNA virus with similarities to Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1. / Du, Zhenguo; Tang, Yafei; Zhang, Songbai; She, Xiaoman; Lan, Guobing; Varsani, Arvind; He, Zifu.

In: Archives of Virology, Vol. 159, No. 6, 2014, p. 1527-1531.

Research output: Contribution to journalArticle

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abstract = "A putative circular single-stranded DNA (ssDNA) virus was recovered from Hypericum japonicum collected in Vietnam. The viral isolate was tentatively named Hypericum japonicum-associated circular DNA virus (HJasCV). HJasCV shares 58.7-65.4{\%} nucleotide sequence identity with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) and SsHADV-1-like viruses. Like this group of viruses, the genome of HJasCV (2 200 nt) has two large ORFs, one in the virion-sense and the other in the complementary-sense DNA. The proteins encoded in the virion-sense and complementary-sense ORFs share 39-46 {\%} and 45-67 {\%} amino acid sequence identity with the putative capsid and replication-associated proteins (Reps), respectively, of SsHADV-1 and SsHADV-1-like viruses. The putative Rep of HJasCV contains all of the motifs related to rolling-circle replication. Its 111-bp intergenic region (IR) contains a hairpin structure with a geminivirus-like nonanucleotide sequence, TAATGTTAT, at the apex of the loop. Phylogenetic analysis revealed that HJasCV forms a monophyletic clade with SsHADV-1 and SsHADV-1-like viruses.",
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