Abstract

Methane oxidation coupled to denitrification (MOD) was tested in a membrane biofilm reactor (MBfR) using methane gas as the sole electron donor. Nitrate reduction to nitrite was rate limiting, and CH4 was present in the effluent. Slow kinetics of methane oxidation by bacteria were the factors that led to slow kinetics and incomplete removals. Methylocystaceae contained the largest fraction (21%) of bacterial SSU rRNA genes, and Archaea were nearly absent. The functional metagenome included all the genes essential for aerobic methane oxidation (pmo, mdh, mtdB, folD, and fdh) and nitrate reduction to dinitrogen (nap/nar, nir, nor and nos), but not for reverse methanogenesis (mcr). The functional metagenome supports that Methylocystaceae conducted MOD in syntrophy with heterotrophic denitrifiers (e.g., Comamonadaceae and Brucellaceae), suggesting aerobic MOD. DO measurements, serum-bottle tests, and calculation of O2 permeation bolster hypoxically aerobic MOD would mainly account for denitrification in the MBfR.

Original languageEnglish (US)
Pages (from-to)745-753
Number of pages9
JournalChemical Engineering Journal
Volume348
DOIs
StatePublished - Sep 15 2018

Fingerprint

Denitrification
Methane
Biofilms
biofilm
denitrification
methane
membrane
Membranes
oxidation
Oxidation
Nitrates
Genes
nitrate
kinetics
Kinetics
gene
methanogenesis
Bottles
Nitrites
Permeation

Keywords

  • Denitrification
  • Dissolved methane
  • Metagenome
  • Methane oxidation
  • Methanotrophs
  • Methylocystaceae

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Hypoxic methane oxidation coupled to denitrification in a membrane biofilm. / Alrashed, Wael; Lee, Jangho; Park, Joonhong; Rittmann, Bruce; Tang, Youneng; Neufeld, Josh D.; Lee, Hyung Sool.

In: Chemical Engineering Journal, Vol. 348, 15.09.2018, p. 745-753.

Research output: Contribution to journalArticle

Alrashed, Wael ; Lee, Jangho ; Park, Joonhong ; Rittmann, Bruce ; Tang, Youneng ; Neufeld, Josh D. ; Lee, Hyung Sool. / Hypoxic methane oxidation coupled to denitrification in a membrane biofilm. In: Chemical Engineering Journal. 2018 ; Vol. 348. pp. 745-753.
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