Hyphal tip cytoplasmic organization in four zygomycetous fungi

Karen E. Fisher, Robert Roberson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.

Original languageEnglish (US)
Pages (from-to)533-542
Number of pages10
JournalMycologia
Volume108
Issue number3
DOIs
StatePublished - May 1 2016

Fingerprint

vesicle
Transmission Electron Microscopy
Mortierellales
Coemansia
Kickxellales
transmission electron microscopy
Fungi
Mucorales
Mortierella
Freeze Substitution
fungus
Mucor
fungi
Hyphae
secretory granules
Cryopreservation
Secretory Vesicles
cryopreservation
hyphae
preserves

Keywords

  • Apical vesicle crescent
  • Cryofixation
  • Electron microscopy
  • Hyphal growth
  • Light microscopy
  • Phylogeny
  • Vesicle organization

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Physiology

Cite this

Hyphal tip cytoplasmic organization in four zygomycetous fungi. / Fisher, Karen E.; Roberson, Robert.

In: Mycologia, Vol. 108, No. 3, 01.05.2016, p. 533-542.

Research output: Contribution to journalArticle

Fisher, Karen E. ; Roberson, Robert. / Hyphal tip cytoplasmic organization in four zygomycetous fungi. In: Mycologia. 2016 ; Vol. 108, No. 3. pp. 533-542.
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