Hydrogen peroxide as an alternate substrate for the oxygen-evolving complex

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Abstract

Photosystem II reaction centers evolve O2 in the dark when H2O2 is added as a substrate. Although some of this activity can be attributed to catalase, as much as 75% of the activity was not affected by the addition of 1 mM KCN. Several lines of evidence demonstrate that this KCN-insensitive O2 evolution from H2O2 in the dark is catalyzed by the cycling of S states in the oxygen-evolving complex including: (1) inactivation of H2O2-mediated O2 evolution by Ca/EDTA washing; (2) susceptibility of the activity to inhibition by amines like ammonia and Tris; (3) inhibition by CCCP which is known to accelerate the rate of deactivation of the S2 state and; (4) a direct dependence of the rate of O2 evolution on the presence of calcium and (5) chloride.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalBBA - Bioenergetics
Volume891
Issue number1
DOIs
StatePublished - Mar 25 1987
Externally publishedYes

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Keywords

  • Hydrogen peroxide
  • Oxygen evolution
  • Oxygen-evolving complex
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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