Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs

Luiz E. Bertassoni, Martina Cecconi, Vijayan Manoharan, Mehdi Nikkhah, Jesper Hjortnaes, Ana Luiza Cristino, Giada Barabaschi, Danilo Demarchi, Mehmet R. Dokmeci, Yunzhi Yang, Ali Khademhosseini

Research output: Contribution to journalArticle

350 Citations (Scopus)

Abstract

Vascularization remains a critical challenge in tissue engineering. The development of vascular networks within densely populated and metabolically functional tissues facilitate transport of nutrients and removal of waste products, thus preserving cellular viability over a long period of time. Despite tremendous progress in fabricating complex tissue constructs in the past few years, approaches for controlled vascularization within hydrogel based engineered tissue constructs have remained limited. Here, we report a three dimensional (3D) micromolding technique utilizing bioprinted agarose template fibers to fabricate microchannel networks with various architectural features within photocrosslinkable hydrogel constructs. Using the proposed approach, we were able to successfully embed functional and perfusable microchannels inside methacrylated gelatin (GelMA), star poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol) diacrylate (PEGDA) hydrogels at different concentrations. In particular, GelMA hydrogels were used as a model to demonstrate the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. In addition, successful formation of endothelial monolayers within the fabricated channels was confirmed. Overall, our proposed strategy represents an effective technique for vascularization of hydrogel constructs with useful applications in tissue engineering and organs on a chip.

Original languageEnglish (US)
Pages (from-to)2202-2211
Number of pages10
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume14
Issue number13
DOIs
StatePublished - Jul 7 2014

Fingerprint

Hydrogel
Tissue Engineering
Microchannels
Tissue engineering
Hydrogels
Tissue
Polyethylene glycols
Blood Vessels
Waste Products
Ethylene Glycol
Gelatin
Sepharose
Nutrients
Stars
Cell Differentiation
Monolayers
Mass transfer
Food
Fibers

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Bertassoni, L. E., Cecconi, M., Manoharan, V., Nikkhah, M., Hjortnaes, J., Cristino, A. L., ... Khademhosseini, A. (2014). Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab on a Chip - Miniaturisation for Chemistry and Biology, 14(13), 2202-2211. https://doi.org/10.1039/c4lc00030g

Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. / Bertassoni, Luiz E.; Cecconi, Martina; Manoharan, Vijayan; Nikkhah, Mehdi; Hjortnaes, Jesper; Cristino, Ana Luiza; Barabaschi, Giada; Demarchi, Danilo; Dokmeci, Mehmet R.; Yang, Yunzhi; Khademhosseini, Ali.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 13, 07.07.2014, p. 2202-2211.

Research output: Contribution to journalArticle

Bertassoni, LE, Cecconi, M, Manoharan, V, Nikkhah, M, Hjortnaes, J, Cristino, AL, Barabaschi, G, Demarchi, D, Dokmeci, MR, Yang, Y & Khademhosseini, A 2014, 'Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 14, no. 13, pp. 2202-2211. https://doi.org/10.1039/c4lc00030g
Bertassoni, Luiz E. ; Cecconi, Martina ; Manoharan, Vijayan ; Nikkhah, Mehdi ; Hjortnaes, Jesper ; Cristino, Ana Luiza ; Barabaschi, Giada ; Demarchi, Danilo ; Dokmeci, Mehmet R. ; Yang, Yunzhi ; Khademhosseini, Ali. / Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2014 ; Vol. 14, No. 13. pp. 2202-2211.
@article{4af8629888eb4a06997df444cdfc9acb,
title = "Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs",
abstract = "Vascularization remains a critical challenge in tissue engineering. The development of vascular networks within densely populated and metabolically functional tissues facilitate transport of nutrients and removal of waste products, thus preserving cellular viability over a long period of time. Despite tremendous progress in fabricating complex tissue constructs in the past few years, approaches for controlled vascularization within hydrogel based engineered tissue constructs have remained limited. Here, we report a three dimensional (3D) micromolding technique utilizing bioprinted agarose template fibers to fabricate microchannel networks with various architectural features within photocrosslinkable hydrogel constructs. Using the proposed approach, we were able to successfully embed functional and perfusable microchannels inside methacrylated gelatin (GelMA), star poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol) diacrylate (PEGDA) hydrogels at different concentrations. In particular, GelMA hydrogels were used as a model to demonstrate the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. In addition, successful formation of endothelial monolayers within the fabricated channels was confirmed. Overall, our proposed strategy represents an effective technique for vascularization of hydrogel constructs with useful applications in tissue engineering and organs on a chip.",
author = "Bertassoni, {Luiz E.} and Martina Cecconi and Vijayan Manoharan and Mehdi Nikkhah and Jesper Hjortnaes and Cristino, {Ana Luiza} and Giada Barabaschi and Danilo Demarchi and Dokmeci, {Mehmet R.} and Yunzhi Yang and Ali Khademhosseini",
year = "2014",
month = "7",
day = "7",
doi = "10.1039/c4lc00030g",
language = "English (US)",
volume = "14",
pages = "2202--2211",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "13",

}

TY - JOUR

T1 - Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs

AU - Bertassoni, Luiz E.

AU - Cecconi, Martina

AU - Manoharan, Vijayan

AU - Nikkhah, Mehdi

AU - Hjortnaes, Jesper

AU - Cristino, Ana Luiza

AU - Barabaschi, Giada

AU - Demarchi, Danilo

AU - Dokmeci, Mehmet R.

AU - Yang, Yunzhi

AU - Khademhosseini, Ali

PY - 2014/7/7

Y1 - 2014/7/7

N2 - Vascularization remains a critical challenge in tissue engineering. The development of vascular networks within densely populated and metabolically functional tissues facilitate transport of nutrients and removal of waste products, thus preserving cellular viability over a long period of time. Despite tremendous progress in fabricating complex tissue constructs in the past few years, approaches for controlled vascularization within hydrogel based engineered tissue constructs have remained limited. Here, we report a three dimensional (3D) micromolding technique utilizing bioprinted agarose template fibers to fabricate microchannel networks with various architectural features within photocrosslinkable hydrogel constructs. Using the proposed approach, we were able to successfully embed functional and perfusable microchannels inside methacrylated gelatin (GelMA), star poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol) diacrylate (PEGDA) hydrogels at different concentrations. In particular, GelMA hydrogels were used as a model to demonstrate the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. In addition, successful formation of endothelial monolayers within the fabricated channels was confirmed. Overall, our proposed strategy represents an effective technique for vascularization of hydrogel constructs with useful applications in tissue engineering and organs on a chip.

AB - Vascularization remains a critical challenge in tissue engineering. The development of vascular networks within densely populated and metabolically functional tissues facilitate transport of nutrients and removal of waste products, thus preserving cellular viability over a long period of time. Despite tremendous progress in fabricating complex tissue constructs in the past few years, approaches for controlled vascularization within hydrogel based engineered tissue constructs have remained limited. Here, we report a three dimensional (3D) micromolding technique utilizing bioprinted agarose template fibers to fabricate microchannel networks with various architectural features within photocrosslinkable hydrogel constructs. Using the proposed approach, we were able to successfully embed functional and perfusable microchannels inside methacrylated gelatin (GelMA), star poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol) diacrylate (PEGDA) hydrogels at different concentrations. In particular, GelMA hydrogels were used as a model to demonstrate the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. In addition, successful formation of endothelial monolayers within the fabricated channels was confirmed. Overall, our proposed strategy represents an effective technique for vascularization of hydrogel constructs with useful applications in tissue engineering and organs on a chip.

UR - http://www.scopus.com/inward/record.url?scp=84901915693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901915693&partnerID=8YFLogxK

U2 - 10.1039/c4lc00030g

DO - 10.1039/c4lc00030g

M3 - Article

C2 - 24860845

AN - SCOPUS:84901915693

VL - 14

SP - 2202

EP - 2211

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 13

ER -