Hybrid TiO 2 nanoparticles: An approach for developing site specific DNA cleavage

J. Liu, Z. Saponjic, N. M. Dimitrijevic, S. Luo, D. Preuss, T. Rajh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We have developed hybrid light responsive TiO 2 nanoparticles electronically linked to PNA oligonucleotides that site specifically bind to double stranded target DNA. This opens a new opportunity for the development of a highly efficient "artificial restriction enzyme" whose activity can be controlled by using light. The work focuses on the use of TiO 2 nanocomposites as analogs of restriction enzymes with unique specificity that does not exist in current biological approaches. TiO 2 nanoparticles electronically linked to DNA or PNA adapters have been site-specifically attached along double stranded X DNA vectors. Illumination of this assembly results in selective oxidation of DNA at the deepest "thermodynamic traps" located closest to the nanoparticle surface, causing DNA cleavage. We investigate the effect of the sequence and length of DNA and PNA adapters on the specificity of DNA cleavage. Related to this issue, the potential use of TiO 2/DNA nanocomposites as "rare cutters" that cleave DNA in the places not achieved with existing protein-based enzymes is investigated.

Original languageEnglish (US)
Title of host publicationColloidal Quantum Dots for Biomedical Applications
DOIs
StatePublished - 2006
Externally publishedYes
EventColloidal Quantum Dots for Biomedical Applications - San Jose, CA, United States
Duration: Jan 22 2006Jan 24 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6096
ISSN (Print)1605-7422

Conference

ConferenceColloidal Quantum Dots for Biomedical Applications
Country/TerritoryUnited States
CitySan Jose, CA
Period1/22/061/24/06

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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