@inproceedings{48ced90292f44507b2cf72dae455f5af,
title = "Hybrid TiO 2 nanoparticles: An approach for developing site specific DNA cleavage",
abstract = "We have developed hybrid light responsive TiO 2 nanoparticles electronically linked to PNA oligonucleotides that site specifically bind to double stranded target DNA. This opens a new opportunity for the development of a highly efficient {"}artificial restriction enzyme{"} whose activity can be controlled by using light. The work focuses on the use of TiO 2 nanocomposites as analogs of restriction enzymes with unique specificity that does not exist in current biological approaches. TiO 2 nanoparticles electronically linked to DNA or PNA adapters have been site-specifically attached along double stranded X DNA vectors. Illumination of this assembly results in selective oxidation of DNA at the deepest {"}thermodynamic traps{"} located closest to the nanoparticle surface, causing DNA cleavage. We investigate the effect of the sequence and length of DNA and PNA adapters on the specificity of DNA cleavage. Related to this issue, the potential use of TiO 2/DNA nanocomposites as {"}rare cutters{"} that cleave DNA in the places not achieved with existing protein-based enzymes is investigated.",
author = "J. Liu and Z. Saponjic and Dimitrijevic, {N. M.} and S. Luo and D. Preuss and T. Rajh",
year = "2006",
doi = "10.1117/12.661778",
language = "English (US)",
isbn = "0819461385",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Colloidal Quantum Dots for Biomedical Applications",
note = "Colloidal Quantum Dots for Biomedical Applications ; Conference date: 22-01-2006 Through 24-01-2006",
}