TY - GEN
T1 - Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture
AU - Christen, Jennifer Blain
AU - Andreou, Andreas G.
PY - 2006
Y1 - 2006
N2 - We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone PDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.
AB - We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone PDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.
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M3 - Conference contribution
AN - SCOPUS:34547369021
SN - 0780393902
SN - 9780780393905
T3 - Proceedings - IEEE International Symposium on Circuits and Systems
SP - 1135
EP - 1138
BT - ISCAS 2006
T2 - ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems
Y2 - 21 May 2006 through 24 May 2006
ER -