Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture

Jennifer Blain Christen, Andreas G. Andreou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone PDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.

Original languageEnglish (US)
Title of host publicationProceedings - IEEE International Symposium on Circuits and Systems
Pages1135-1138
Number of pages4
StatePublished - 2006
Externally publishedYes
EventISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems - Kos, Greece
Duration: May 21 2006May 24 2006

Other

OtherISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems
CountryGreece
CityKos
Period5/21/065/24/06

Fingerprint

Microsystems
Fluidics
Silicon
Silicones
Polydimethylsiloxane
Cell culture
Flat cables
Temperature sensors
Polyimides
Packaging
Multilayers
Gases
Impurities
Membranes
Fabrication
Liquids
Testing
baysilon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

Cite this

Blain Christen, J., & Andreou, A. G. (2006). Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture. In Proceedings - IEEE International Symposium on Circuits and Systems (pp. 1135-1138). [1692790]

Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture. / Blain Christen, Jennifer; Andreou, Andreas G.

Proceedings - IEEE International Symposium on Circuits and Systems. 2006. p. 1135-1138 1692790.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Blain Christen, J & Andreou, AG 2006, Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture. in Proceedings - IEEE International Symposium on Circuits and Systems., 1692790, pp. 1135-1138, ISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems, Kos, Greece, 5/21/06.
Blain Christen J, Andreou AG. Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture. In Proceedings - IEEE International Symposium on Circuits and Systems. 2006. p. 1135-1138. 1692790
Blain Christen, Jennifer ; Andreou, Andreas G. / Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture. Proceedings - IEEE International Symposium on Circuits and Systems. 2006. pp. 1135-1138
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