Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture

Jennifer Blain Christen, Andreas G. Andreou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone FDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.

Original languageEnglish (US)
Title of host publication28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Pages2490-2493
Number of pages4
DOIs
StatePublished - Dec 1 2006
Externally publishedYes
Event28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States
Duration: Aug 30 2006Sep 3 2006

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)0589-1019

Other

Other28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
CountryUnited States
CityNew York, NY
Period8/30/069/3/06

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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