Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells: Potential for clinical application

Michael S. Von Bergwelt-Baildon, Robert H. Vonderheide, Britta Maecker, Naoto Hirano, Karen Anderson, Marcus O. Butler, Zhinan Xia, Wan Y. Zeng, Kai W. Wucherpfennig, Lee M. Nadler, Joachim L. Schultze

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

CD40 engagement is the major signal that induces B cells to efficiently present antigen to T cells. We previously demonstrated that human peripheral blood-derived CD40-activated B cells (CD40-B cells) function as antigen-presenting cells (APCs). Here, we have established a culture system to generate these APCs under clinically applicable conditions using guanylic acid-grade soluble trimeric CD40 ligand. To monitor APC function and antigen loading for these cells, simple and efficient quality control assays have been developed. Using this approach, we demonstrate that CD40-B cells from healthy donors and cancer patients are fully functional and equally expanded in long-term cultures. These B cells boost robust memory T-cell responses, but more importantly, they also prime naive T-cell responses against neoantigens ex vivo. CD40-B cells overcome current obstacles, such as the difficulty of isolation, generation, and long-term expansion observed with other APCs. Therefore, they are an excellent source of professional APCs for immune assessment, antigen discovery, and antigen-specific immunotherapy.

Original languageEnglish (US)
Pages (from-to)3319-3325
Number of pages7
JournalBlood
Volume99
Issue number9
DOIs
StatePublished - May 1 2002
Externally publishedYes

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T-cells
Cytotoxic T-Lymphocytes
Antigen-Presenting Cells
B-Lymphocytes
Cells
Data storage equipment
Antigens
T-Lymphocytes
Guanosine Monophosphate
CD40 Ligand
Cell culture
Quality Control
Immunotherapy
Quality control
Assays
Blood
Tissue Donors
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells : Potential for clinical application. / Von Bergwelt-Baildon, Michael S.; Vonderheide, Robert H.; Maecker, Britta; Hirano, Naoto; Anderson, Karen; Butler, Marcus O.; Xia, Zhinan; Zeng, Wan Y.; Wucherpfennig, Kai W.; Nadler, Lee M.; Schultze, Joachim L.

In: Blood, Vol. 99, No. 9, 01.05.2002, p. 3319-3325.

Research output: Contribution to journalArticle

Von Bergwelt-Baildon, MS, Vonderheide, RH, Maecker, B, Hirano, N, Anderson, K, Butler, MO, Xia, Z, Zeng, WY, Wucherpfennig, KW, Nadler, LM & Schultze, JL 2002, 'Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells: Potential for clinical application', Blood, vol. 99, no. 9, pp. 3319-3325. https://doi.org/10.1182/blood.V99.9.3319
Von Bergwelt-Baildon, Michael S. ; Vonderheide, Robert H. ; Maecker, Britta ; Hirano, Naoto ; Anderson, Karen ; Butler, Marcus O. ; Xia, Zhinan ; Zeng, Wan Y. ; Wucherpfennig, Kai W. ; Nadler, Lee M. ; Schultze, Joachim L. / Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells : Potential for clinical application. In: Blood. 2002 ; Vol. 99, No. 9. pp. 3319-3325.
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