Human hepatocellular carcinoma is characterized by a highly consistent pattern of genomic imbalances, including frequent loss of 16q23.1-24.1

Binaifer R. Balsara, Jianming Pei, Assunta De Rienzo, Daniela Simon, Alessandra Tosolini, You Yong Lu, Fu Min Shen, Xianglin Fan, Wen Yao Lin, Kenneth H. Buetow, W. Thomas London, Joseph R. Testa

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Comparative genomic hybridization (CGH) analysis was used to identify chromosomal imbalances in 52 human primary hepatocellular carcinomas (HCCs). The most prominent changes were gains of part or all of chromosome arms 8q (83% of cases) and Iq (73%) and loss of 16q (63%). Other commonly overrepresented sites were 5p, 7q, and Xq. Recurrent sites of DNA sequence amplification included 8q23-24 (five cases) and 11q13-14 (four cases). Other frequently underrepresented sites were 4q, 8p, 16p, and 17p. Taken collectively, these findings and data from other CGH studies of HCCs define a subset of chromosome segments that are consistently over- or underrepresented and highlight sites of putative oncogenes and tumor suppressor genes, respectively, involved in hepatocellular oncogenesis. Loss of heterozygosity analysis with a panel of polymorphic microsatellite markers distributed along 16q defined a minimal region of chromosomal loss at 16q23.1-24.1, suggesting that this region harbors a tumor suppressor gene whose loss/inactivation may contribute to the pathogenesis of many HCCs.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalGenes Chromosomes and Cancer
Volume30
Issue number3
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Human hepatocellular carcinoma is characterized by a highly consistent pattern of genomic imbalances, including frequent loss of 16q23.1-24.1'. Together they form a unique fingerprint.

Cite this