Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain

Thomas J. Pisano, Zahra M. Dhanerawala, Mikhail Kislin, Dariya Bakshinskaya, Esteban A. Engel, Ethan J. Hansen, Austin T. Hoag, Junuk Lee, Nina L. de Oude, Kannan Umadevi Venkataraju, Jessica L. Verpeut, Freek E. Hoebeek, Ben D. Richardson, Henk Jan Boele, Samuel S.H. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebellar outputs take polysynaptic routes to reach the rest of the brain, impeding conventional tracing. Here, we quantify pathways between the cerebellum and forebrain by using transsynaptic tracing viruses and a whole-brain analysis pipeline. With retrograde tracing, we find that most descending paths originate from the somatomotor cortex. Anterograde tracing of ascending paths encompasses most thalamic nuclei, especially ventral posteromedial, lateral posterior, mediodorsal, and reticular nuclei. In the neocortex, sensorimotor regions contain the most labeled neurons, but we find higher densities in associative areas, including orbital, anterior cingulate, prelimbic, and infralimbic cortex. Patterns of ascending expression correlate with c-Fos expression after optogenetic inhibition of Purkinje cells. Our results reveal homologous networks linking single areas of the cerebellar cortex to diverse forebrain targets. We conclude that shared areas of the cerebellum are positioned to provide sensory-motor information to regions implicated in both movement and nonmotor function.

Original languageEnglish (US)
Article number109721
JournalCell Reports
Volume36
Issue number12
DOIs
StatePublished - Sep 21 2021

Keywords

  • cerebellocortical
  • cerebellothalamic
  • corticocerebellar
  • herpes simplex
  • iDISCO
  • light-sheet microscopy
  • nonmotor cerebellum
  • pseudorabies
  • transsynaptic tracing
  • whole-brain connectivity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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