Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps

Richard Gerkin, David W. Nauen, Fang Xu, Guo Qiang Bi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: During development both Hebbian and homeostatic mechanisms regulate synaptic efficacy, usually working in opposite directions in response to neuronal activity. Homeostatic plasticity has often been investigated by assaying changes in spontaneous synaptic transmission resulting from chronic circuit inactivation. However, effects of inactivation on evoked transmission have been less frequently reported. Importantly, contributions from the effects of circuit inactivation and reactivation on synaptic efficacy have not been individuated. Results: Here we show for developing hippocampal neurons in primary culture that chronic inactivation with TTX results in increased mean amplitude of miniature synaptic currents (mEPSCs), but not evoked synaptic currents (eEPSCs). However, changes in quantal properties of transmission, partially reflected in mEPSCs, accurately predicted higher-order statistical properties of eEPSCs. The classical prediction of homeostasis - increased strength of evoked transmission - was realized after explicit circuit reactivation, in the form of cells' pairwise connection probability. In contrast, distributions of eEPSC amplitudes for control and inactivated-then- reactivated groups matched throughout. Conclusions: Homeostatic up-regulation of evoked synaptic transmission in developing hippocampal neurons in primary culture requires both the inactivation and reactivation stages, leading to a net increase in functional circuit connectivity.

Original languageEnglish (US)
Article number38
JournalMolecular Brain
Volume6
Issue number1
DOIs
StatePublished - Aug 26 2013

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Synaptic Transmission
Neurons
Homeostasis
Research Design
Up-Regulation
Direction compound

Keywords

  • Homeostasis
  • Metaplasticity
  • Quantal hypothesis

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology
  • Medicine(all)

Cite this

Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps. / Gerkin, Richard; Nauen, David W.; Xu, Fang; Bi, Guo Qiang.

In: Molecular Brain, Vol. 6, No. 1, 38, 26.08.2013.

Research output: Contribution to journalArticle

Gerkin, Richard ; Nauen, David W. ; Xu, Fang ; Bi, Guo Qiang. / Homeostatic regulation of spontaneous and evoked synaptic transmission in two steps. In: Molecular Brain. 2013 ; Vol. 6, No. 1.
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