Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles

Sean M. Griffing, Giselle M Rachid Viana, Tonya Mixson-Hayden, Sankar Sridaran, Mohammad Tauqeer Alam, Alexandre Macedo de Oliveira, John W. Barnwell, Ananias A. Escalante, Marinete Marins Povoa, Venkatachalam Udhayakumar

Research output: Contribution to journalArticle

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Abstract

Previous work suggests that Brazilian Plasmodium falciparum has limited genetic diversity and a history of bottlenecks, multiple reintroductions due to human migration, and clonal expansions. We hypothesized that Brazilian P. falciparum would exhibit clonal structure. We examined isolates collected across two decades from Amapá, Rondônia, and Pará state (n = 190). By examining more microsatellites markers on more chromosomes than previous studies, we hoped to define the extent of low diversity, linkage disequilibrium, bottlenecks, population structure, and parasite migration within Brazil. We used retrospective genotyping of samples from the 1980s and 1990s to explore the population genetics of SP resistant dhfr and dhps alleles. We tested an existing hypothesis that the triple mutant dhfr mutations 50R/51I/108N and 51I/108N/164L developed in southern Amazon from a single origin of common or similar parasites. We found that Brazilian P. falciparum had limited genetic diversity and isolation by distance was rejected, which suggests it underwent bottlenecks followed by migration between sites. Unlike Peru, there appeared to be gene flow across the Brazilian Amazon basin. We were unable to divide parasite populations by clonal lineages and pairwise FST were common. Most parasite diversity was found within sites in the Brazilian Amazon, according to AMOVA. Our results challenge the hypothesis that triple mutant alleles arose from a single lineage in the Southern Amazon. SP resistance, at both the double and triple mutant stages, developed twice and potentially in different regions of the Brazilian Amazon. We would have required samples from before the 1980s to describe how SP resistance spread across the basin or describe the complex internal migration of Brazilian parasites after the colonization efforts of past decades. The Brazilian Amazon basin may have sufficient internal migration for drug resistance reported in any particular region to rapidly spread to other parts of basin under similar drug pressure.

Original languageEnglish (US)
Article numbere58984
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 15 2013

Fingerprint

drug resistance
Drug Resistance
population structure
Parasites
Alleles
alleles
Plasmodium falciparum
parasites
basins
Pharmaceutical Preparations
Population
mutants
Human Migration
genetic variation
Brazil
Peru
Gene Flow
Linkage Disequilibrium
Population Genetics
Chromosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Griffing, S. M., Viana, G. M. R., Mixson-Hayden, T., Sridaran, S., Alam, M. T., de Oliveira, A. M., ... Udhayakumar, V. (2013). Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles. PLoS One, 8(3), [e58984]. https://doi.org/10.1371/journal.pone.0058984

Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles. / Griffing, Sean M.; Viana, Giselle M Rachid; Mixson-Hayden, Tonya; Sridaran, Sankar; Alam, Mohammad Tauqeer; de Oliveira, Alexandre Macedo; Barnwell, John W.; Escalante, Ananias A.; Povoa, Marinete Marins; Udhayakumar, Venkatachalam.

In: PLoS One, Vol. 8, No. 3, e58984, 15.03.2013.

Research output: Contribution to journalArticle

Griffing, SM, Viana, GMR, Mixson-Hayden, T, Sridaran, S, Alam, MT, de Oliveira, AM, Barnwell, JW, Escalante, AA, Povoa, MM & Udhayakumar, V 2013, 'Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles', PLoS One, vol. 8, no. 3, e58984. https://doi.org/10.1371/journal.pone.0058984
Griffing SM, Viana GMR, Mixson-Hayden T, Sridaran S, Alam MT, de Oliveira AM et al. Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles. PLoS One. 2013 Mar 15;8(3). e58984. https://doi.org/10.1371/journal.pone.0058984
Griffing, Sean M. ; Viana, Giselle M Rachid ; Mixson-Hayden, Tonya ; Sridaran, Sankar ; Alam, Mohammad Tauqeer ; de Oliveira, Alexandre Macedo ; Barnwell, John W. ; Escalante, Ananias A. ; Povoa, Marinete Marins ; Udhayakumar, Venkatachalam. / Historical Shifts in Brazilian P. falciparum Population Structure and Drug Resistance Alleles. In: PLoS One. 2013 ; Vol. 8, No. 3.
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