Histopathologic correlates of serum amylase activity in acute experimental pancreatitis

J. Schmidt, K. Lewandrowski, C. Fernandez Del Castillo, U. Mandavilli, Carolyn Compton, A. L. Warshaw, D. W. Rattner

Research output: Contribution to journalArticle

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Abstract

The association of serum amylase activity with the extent of pancreatic injury in acute pancreatitis is unclear. To clarify this relationship, we induced acute pancreatitis ranging from mild to lethal in 118 Sprague-Dawley rats (350-450 g). This was achieved by controlled intraductal infusion of low- or high-dose bile salt, with or without enterokinase, followed by intravenous cerulein or saline for 6 hr. Serum amylase was measured at baseline and 6 hr. Pancreatic histopathology was evaluated by two blinded pathologists employing total surface scoring (N=118) and morphometric 20-field documentation (N =22). Serum amylase correlated best with edema (r=0.61) and fat necrosis (r=0.58), less well with acinar necrosis (r=0.53) and inflammation (r=0.50), and poorly with hemorrhage (r=0.33) and perivascular infiltrate (r=0.31). Inasmuch as edema and fat necrosis are not important determinants of severity, these observations could explain the poor prognostic value of serum amylase activity in patients with acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)1426-1433
Number of pages8
JournalDigestive Diseases and Sciences
Volume37
Issue number9
DOIs
StatePublished - Sep 1992
Externally publishedYes

Fingerprint

Amylases
Pancreatitis
Fat Necrosis
Serum
Edema
Enteropeptidase
Ceruletide
Bile Acids and Salts
Documentation
Sprague Dawley Rats
Necrosis
Hemorrhage
Inflammation
Wounds and Injuries

Keywords

  • acute pancreatitis
  • correlation
  • experimental pancreatitis
  • histopathology
  • serum amylase

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Schmidt, J., Lewandrowski, K., Castillo, C. F. D., Mandavilli, U., Compton, C., Warshaw, A. L., & Rattner, D. W. (1992). Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. Digestive Diseases and Sciences, 37(9), 1426-1433. https://doi.org/10.1007/BF01296014

Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. / Schmidt, J.; Lewandrowski, K.; Castillo, C. Fernandez Del; Mandavilli, U.; Compton, Carolyn; Warshaw, A. L.; Rattner, D. W.

In: Digestive Diseases and Sciences, Vol. 37, No. 9, 09.1992, p. 1426-1433.

Research output: Contribution to journalArticle

Schmidt, J, Lewandrowski, K, Castillo, CFD, Mandavilli, U, Compton, C, Warshaw, AL & Rattner, DW 1992, 'Histopathologic correlates of serum amylase activity in acute experimental pancreatitis', Digestive Diseases and Sciences, vol. 37, no. 9, pp. 1426-1433. https://doi.org/10.1007/BF01296014
Schmidt, J. ; Lewandrowski, K. ; Castillo, C. Fernandez Del ; Mandavilli, U. ; Compton, Carolyn ; Warshaw, A. L. ; Rattner, D. W. / Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. In: Digestive Diseases and Sciences. 1992 ; Vol. 37, No. 9. pp. 1426-1433.
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