Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun, Shifeng Wang, Roy Curtiss

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

Original languageEnglish (US)
Pages (from-to)4241-4245
Number of pages5
JournalApplied and environmental microbiology
Volume74
Issue number13
DOIs
StatePublished - Jul 2008

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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