Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun; Shifeng Wang; Roy Curtiss

doi:10.1128/AEM.00940-08

Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun, Shifeng Wang, Roy Curtiss

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

Original language	English (US)
Pages (from-to)	4241-4245
Number of pages	5
Journal	Applied and environmental microbiology
Volume	74
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00940-08
State	Published - Jul 2008

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.00940-08

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abstract = "An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.",

author = "Wei Sun and Shifeng Wang and Roy Curtiss",

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Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Abstract

ASJC Scopus subject areas

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