Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Wei Sun, Shifeng Wang, Roy Curtiss

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines γ Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

Original languageEnglish (US)
Pages (from-to)4241-4245
Number of pages5
JournalApplied and Environmental Microbiology
Volume74
Issue number13
DOIs
StatePublished - Jul 2008

Fingerprint

Yersinia pestis
gene deletion
Insertional Mutagenesis
Gene Deletion
live vaccines
genetic engineering
Genetic Recombination
recombination
chromosome
Chromosomes
chromosomes
Attenuated Vaccines
gene
vaccine
genes
screening
methodology
Genes
genetic modification
method

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome. / Sun, Wei; Wang, Shifeng; Curtiss, Roy.

In: Applied and Environmental Microbiology, Vol. 74, No. 13, 07.2008, p. 4241-4245.

Research output: Contribution to journalArticle

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