TY - JOUR
T1 - Highly-efficient enzymatic conversion of crude algal oils into biodiesel
AU - Wang, Yao
AU - Liu, Jin
AU - Gerken, Henri
AU - Zhang, Chengwu
AU - Hu, Qiang
AU - Li, Yantao
N1 - Funding Information:
This project was partly supported by Climate Change and Emissions Management Corporation (CCEMC). Yao Wang was partly sponsored by China Scholarship Council (CSC), Jinan University, and Institute of Marine and Environmental Technology .
Publisher Copyright:
© 2014.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Energy-intensive chemical conversion of crude algal oils into biodiesel is a major barrier for cost-effective algal biofuel production. To overcome this problem, we developed an enzyme-based platform for conversion of crude algal oils into fatty acid methyl esters. Crude algal oils were extracted from the oleaginous microalga Nannochloropsis oceanica IMET1 and converted by an immobilized lipase from Candida antarctica. The effects of different acyl acceptors, t-butanol as a co-solvent, oil to t-butanol ratio, oil to methanol ratio, temperature and reaction time on biodiesel conversion efficiency were studied. The conversion efficiency reached 99.1% when the conversion conditions were optimized, i.e., an oil to t-butanol weight ratio of 1:1, an oil to methanol molar ratio of 1:12, and a reaction time of 4. h at 25. °C. The enzymatic conversion process developed in this study may hold a promise for low energy consumption, low wastewater-discharge biochemical conversion of algal feedstocks into biofuels.
AB - Energy-intensive chemical conversion of crude algal oils into biodiesel is a major barrier for cost-effective algal biofuel production. To overcome this problem, we developed an enzyme-based platform for conversion of crude algal oils into fatty acid methyl esters. Crude algal oils were extracted from the oleaginous microalga Nannochloropsis oceanica IMET1 and converted by an immobilized lipase from Candida antarctica. The effects of different acyl acceptors, t-butanol as a co-solvent, oil to t-butanol ratio, oil to methanol ratio, temperature and reaction time on biodiesel conversion efficiency were studied. The conversion efficiency reached 99.1% when the conversion conditions were optimized, i.e., an oil to t-butanol weight ratio of 1:1, an oil to methanol molar ratio of 1:12, and a reaction time of 4. h at 25. °C. The enzymatic conversion process developed in this study may hold a promise for low energy consumption, low wastewater-discharge biochemical conversion of algal feedstocks into biofuels.
KW - Crude algal oils
KW - Enzymatic conversion
KW - Fatty acid methyl esters
KW - Lipase
KW - Nannochloropsis oceanica IMET1
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U2 - 10.1016/j.biortech.2014.09.003
DO - 10.1016/j.biortech.2014.09.003
M3 - Article
C2 - 25255191
AN - SCOPUS:84907567348
SN - 0960-8524
VL - 172
SP - 143
EP - 149
JO - Bioresource Technology
JF - Bioresource Technology
ER -