High-throughput screening in two dimensions: Binding intensity and off-rate on a peptide microarray

Matthew P. Greving, Paul E. Belcher, Conor D. Cox, Douglas Daniel, Chris Diehnelt, Neal Woodbury

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We report a high-throughput two-dimensional microarray-based screen, incorporating both target binding intensity and off-rate, which can be used to analyze thousands of compounds in a single binding assay. Relative binding intensities and time-resolved dissociation are measured for labeled tumor necrosis factor alpha (TNF-α) bound to a peptide microarray. The time-resolved dissociation is fitted to a one-component exponential decay model, from which relative dissociation rates are determined for all peptides with binding intensities above background. We show that most peptides with the slowest off-rates on the microarray also have the slowest off-rates when measured by surface plasmon resonance (SPR).

Original languageEnglish (US)
Pages (from-to)93-95
Number of pages3
JournalAnalytical Biochemistry
Volume402
Issue number1
DOIs
StatePublished - Jul 1 2010

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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