HIGH throughput nanoparticle sorting for serial femtosecond crystallography

B. G. Abdallah, M. Sawtelle, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a high throughput microfluidic method for isolating nanoparticles to aid in protein structure elucidation via serial femtosecond crystallography (SFX). This work improves upon our existing sorter for uniform crystal fractionation by increasing throughput and reducing experimentation time by a factor > 25. Numerical simulations are presented, which were used to optimize the second generation device design, as well as demonstrate the possibility to sort nanoparticles from microparticles using insulator-based dielectrophoresis (iDEP). Additionally, we illustrate the possibilities of variable size selectivity and increased nanoparticle recovery by coupling two devices in serial.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1305-1307
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Dielectrophoresis
  • Microfluidics
  • Nanoparticles
  • Sorting

ASJC Scopus subject areas

  • Control and Systems Engineering

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