Abstract
We propose a high throughput microfluidic method for isolating nanoparticles to aid in protein structure elucidation via serial femtosecond crystallography (SFX). This work improves upon our existing sorter for uniform crystal fractionation by increasing throughput and reducing experimentation time by a factor > 25. Numerical simulations are presented, which were used to optimize the second generation device design, as well as demonstrate the possibility to sort nanoparticles from microparticles using insulator-based dielectrophoresis (iDEP). Additionally, we illustrate the possibilities of variable size selectivity and increased nanoparticle recovery by coupling two devices in serial.
| Original language | English (US) |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1305-1307 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
Keywords
- Dielectrophoresis
- Microfluidics
- Nanoparticles
- Sorting
ASJC Scopus subject areas
- Control and Systems Engineering