TY - GEN
T1 - High-throughput mutagenized cell screening system capable of selective single cell extraction
AU - Kim, Hyun Soo
AU - Weiss, Taylor L.
AU - Devarrenne, Timothy P.
AU - Han, Arum
PY - 2012
Y1 - 2012
N2 - We have developed a high-throughput microfluidic screening platform capable of culturing, analyzing, and selectively extracting mutagenized cells to investigate genetic variants. Combination of two pneumatic control layers, each comprising 32 microchannels, enables individually addressing and controlling each of the 1024 trapping sites in the cell analysis layer. By pressurizing either of the two pneumatic control channels or releasing pressure from both channels, the underlying trapping site was opened or closed, respectively. Cells inside a particular trapping site were extracted with backflow by selectively opening only this site. The performance of this screening platform was characterized using green microalga Tetraslimis suecica.
AB - We have developed a high-throughput microfluidic screening platform capable of culturing, analyzing, and selectively extracting mutagenized cells to investigate genetic variants. Combination of two pneumatic control layers, each comprising 32 microchannels, enables individually addressing and controlling each of the 1024 trapping sites in the cell analysis layer. By pressurizing either of the two pneumatic control channels or releasing pressure from both channels, the underlying trapping site was opened or closed, respectively. Cells inside a particular trapping site were extracted with backflow by selectively opening only this site. The performance of this screening platform was characterized using green microalga Tetraslimis suecica.
KW - Microalgae culture microsystem
KW - Microfluidic high-throughput screening platform
KW - Mutagenesis
KW - Photosynthetic microalgae
KW - Selective single cell extraction
UR - http://www.scopus.com/inward/record.url?scp=84901814429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901814429&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901814429
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 506
EP - 508
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -