High-throughput mutagenized cell screening system capable of selective single cell extraction

Hyun Soo Kim, Taylor L. Weiss, Timothy P. Devarrenne, Arum Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a high-throughput microfluidic screening platform capable of culturing, analyzing, and selectively extracting mutagenized cells to investigate genetic variants. Combination of two pneumatic control layers, each comprising 32 microchannels, enables individually addressing and controlling each of the 1024 trapping sites in the cell analysis layer. By pressurizing either of the two pneumatic control channels or releasing pressure from both channels, the underlying trapping site was opened or closed, respectively. Cells inside a particular trapping site were extracted with backflow by selectively opening only this site. The performance of this screening platform was characterized using green microalga Tetraslimis suecica.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages506-508
Number of pages3
ISBN (Print)9780979806452
StatePublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Microalgae culture microsystem
  • Microfluidic high-throughput screening platform
  • Mutagenesis
  • Photosynthetic microalgae
  • Selective single cell extraction

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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