High Throughput Mapping of Antibody Epitopes and Protein Binding Regions by Random Peptides Microarrays

Stephen Johnston (Inventor)

Research output: Patent

Abstract

Current approaches utilized to map antibody epitopes or protein interaction regions requre either a prior knowledge of the general region of interaction or the synthesis of hundreds of short linear peptides that compose the entire protein. This approach is available commercially by Pepscan Systems, B.V. This requires the production of a new peptide library for each antibody or protein analyzed. Our approach requires the synthesis of a single random peptides library for all antibodies and proteins, and as such can be scaled up for high throughput determination of multiple antibody eptiopes in a single experiment.
Original languageEnglish (US)
StatePublished - Mar 24 2006

Fingerprint

Microarrays
Epitopes
Throughput
Peptide Library
Peptides
Antibodies
Proteins
Protein Binding
Experiments

Cite this

@misc{0fbb45812a77461d88fb7280f95b0b40,
title = "High Throughput Mapping of Antibody Epitopes and Protein Binding Regions by Random Peptides Microarrays",
abstract = "Current approaches utilized to map antibody epitopes or protein interaction regions requre either a prior knowledge of the general region of interaction or the synthesis of hundreds of short linear peptides that compose the entire protein. This approach is available commercially by Pepscan Systems, B.V. This requires the production of a new peptide library for each antibody or protein analyzed. Our approach requires the synthesis of a single random peptides library for all antibodies and proteins, and as such can be scaled up for high throughput determination of multiple antibody eptiopes in a single experiment.",
author = "Stephen Johnston",
year = "2006",
month = "3",
day = "24",
language = "English (US)",
type = "Patent",

}

TY - PAT

T1 - High Throughput Mapping of Antibody Epitopes and Protein Binding Regions by Random Peptides Microarrays

AU - Johnston, Stephen

PY - 2006/3/24

Y1 - 2006/3/24

N2 - Current approaches utilized to map antibody epitopes or protein interaction regions requre either a prior knowledge of the general region of interaction or the synthesis of hundreds of short linear peptides that compose the entire protein. This approach is available commercially by Pepscan Systems, B.V. This requires the production of a new peptide library for each antibody or protein analyzed. Our approach requires the synthesis of a single random peptides library for all antibodies and proteins, and as such can be scaled up for high throughput determination of multiple antibody eptiopes in a single experiment.

AB - Current approaches utilized to map antibody epitopes or protein interaction regions requre either a prior knowledge of the general region of interaction or the synthesis of hundreds of short linear peptides that compose the entire protein. This approach is available commercially by Pepscan Systems, B.V. This requires the production of a new peptide library for each antibody or protein analyzed. Our approach requires the synthesis of a single random peptides library for all antibodies and proteins, and as such can be scaled up for high throughput determination of multiple antibody eptiopes in a single experiment.

M3 - Patent

ER -