High-throughput, long-term imaging of Salmonella infecting macrophages in a micro-environmental system

Shih Hui Chao, Tim J. Strovas, Ting She M. Wang, Kendan A. Jones-Isaac, Susan L. Fink, Brad T. Cookson, Deirdre R. Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Real-time single cell analysis is necessary to understand dynamic cellular functions in time and space. Such analyses require the simultaneous measurement of multiple variables in real-time, due to heterogeneity in cellular populations. We report the application of using a micro-environmental chamber on an automatic laser scanning confocal microscope to observe murine macrophage cells in incubation conditions for more than 18 hours. The motorized stage of the microscope was programmed to scan through pre-defined monitoring locations to increase the observation throughput. The acquired images were post-processed to extract the information of each cell. In contrast to current single-cell technologies, such as fluorescence-activated cell sorter (FACS) based systems, the reported architecture records the history of the physiological responses of individual cells.

Original languageEnglish (US)
Title of host publicationProceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
PublisherAmerican Society of Mechanical Engineers
Pages1053-1058
Number of pages6
ISBN (Print)0791847608, 9780791847602
DOIs
StatePublished - 2006
Externally publishedYes
Event4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006 - Limerick, Ireland
Duration: Jun 19 2006Jun 21 2006

Publication series

NameProceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
Volume2006 B

Other

Other4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
CountryIreland
CityLimerick
Period6/19/066/21/06

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Chao, S. H., Strovas, T. J., Wang, T. S. M., Jones-Isaac, K. A., Fink, S. L., Cookson, B. T., & Meldrum, D. R. (2006). High-throughput, long-term imaging of Salmonella infecting macrophages in a micro-environmental system. In Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006 (pp. 1053-1058). (Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006; Vol. 2006 B). American Society of Mechanical Engineers. https://doi.org/10.1115/icnmm2006-96212