High throughput formation and measurement of occlusive thrombosis in porcine blood

Melissa Li, Angela Sodemann, Jeremy Ackerman, Rhett Mayor, Craig Forest

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Understanding and treating cardiovascular disease can benefit greatly from the development of low volume, high throughput tools to study thrombosis. We have designed, fabricated, and validated a microfluidic device to induce shear rates from 4300-7100 s-1 using only 10 mL of whole blood for 4 simultaneous assays to measure occlusive thrombosis optically. Shear rates were confirmed by flow rate and particle imaging velocimetry. Thrombosis measurements showed similar acute growth rates for all shear rates while time to acute growth onset and occlusion depend on initial shear rate. Applications range from unravelling the blood clotting cascade to drug dosage response measurement.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages445-447
Number of pages3
ISBN (Print)9780979806421
StatePublished - Jan 1 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • Blood
  • Microfluidics
  • Platelets
  • Shear
  • Thrombosis

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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