High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Kenneth H. Buetow; Michael Edmonson; Richard MacDonald; Robert Clifford; Ping Yip; Jenny Kelley; Daniel P. Little; Robert Strausberg; Hubert Koester; Charles R. Cantor; Andreas Braun

doi:10.1073/pnas.98.2.581

High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Kenneth H. Buetow, Michael Edmonson, Richard MacDonald, Robert Clifford, Ping Yip, Jenny Kelley, Daniel P. Little, Robert Strausberg, Hubert Koester, Charles R. Cantor, Andreas Braun

Research output: Contribution to journal › Article › peer-review

426 Scopus citations

Abstract

We describe here a system for the rapid identification, assay development, and characterization of gene-based single nucleotide polymorphisms (SNPs). This system couples informatics tools that mine candidate SNPs from public expressed sequence tag resources and automatically designs assay reagents with detection by a chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform. As a proof of concept of this system, a genomewide collection of reagents for 9,115 gene-based SNP genetic markers was rapidly developed and validated. These data provide preliminary insights into patterns of polymorphism in a genomewide collection of gene-based polymorphisms.

Original language	English (US)
Pages (from-to)	581-584
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	2
DOIs	https://doi.org/10.1073/pnas.98.2.581
State	Published - Jan 16 2001
Externally published	Yes

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Buetow, K. H., Edmonson, M., MacDonald, R., Clifford, R., Yip, P., Kelley, J., Little, D. P., Strausberg, R., Koester, H., Cantor, C. R., & Braun, A. (2001). High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America, 98(2), 581-584. https://doi.org/10.1073/pnas.98.2.581

High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. / Buetow, Kenneth H.; Edmonson, Michael; MacDonald, Richard et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 2, 16.01.2001, p. 581-584.

Research output: Contribution to journal › Article › peer-review

Buetow, KH, Edmonson, M, MacDonald, R, Clifford, R, Yip, P, Kelley, J, Little, DP, Strausberg, R, Koester, H, Cantor, CR & Braun, A 2001, 'High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 2, pp. 581-584. https://doi.org/10.1073/pnas.98.2.581

Buetow KH, Edmonson M, MacDonald R, Clifford R, Yip P, Kelley J et al. High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America. 2001 Jan 16;98(2):581-584. doi: 10.1073/pnas.98.2.581

Buetow, Kenneth H. ; Edmonson, Michael ; MacDonald, Richard et al. / High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 2. pp. 581-584.

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abstract = "We describe here a system for the rapid identification, assay development, and characterization of gene-based single nucleotide polymorphisms (SNPs). This system couples informatics tools that mine candidate SNPs from public expressed sequence tag resources and automatically designs assay reagents with detection by a chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform. As a proof of concept of this system, a genomewide collection of reagents for 9,115 gene-based SNP genetic markers was rapidly developed and validated. These data provide preliminary insights into patterns of polymorphism in a genomewide collection of gene-based polymorphisms.",

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High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Abstract

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