High throughput characterization of hemoglobin from neonates using MALDI-TOF MS

Urban A. Kiernan; Peter Williams; Randall W. Nelson

High throughput characterization of hemoglobin from neonates using MALDI-TOF MS

Urban A. Kiernan, Peter Williams, Randall W. Nelson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

MALDI-TOF targets were used to create a high throughput platform capable of characterizing hemoglobin from neonates at a rate of ∼ 100 samples per hour. Ninety-six neonate dried blood blot samples were collected and isolated in individual wells of a 96-well titer plate and robotically processed in parallel acidified hemoglobin solutions. The intact chains of 96 hemoglobin samples were screened. The absence of an additional signal, either split or shifted by ∼-30 Da, indicates that the mutation was present in the first eight residues of the β-chain and due to the β6Glu Val substitution.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	269-270
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

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title = "High throughput characterization of hemoglobin from neonates using MALDI-TOF MS",

abstract = "MALDI-TOF targets were used to create a high throughput platform capable of characterizing hemoglobin from neonates at a rate of ∼ 100 samples per hour. Ninety-six neonate dried blood blot samples were collected and isolated in individual wells of a 96-well titer plate and robotically processed in parallel acidified hemoglobin solutions. The intact chains of 96 hemoglobin samples were screened. The absence of an additional signal, either split or shifted by ∼-30 Da, indicates that the mutation was present in the first eight residues of the β-chain and due to the β6Glu Val substitution.",

author = "Kiernan, {Urban A.} and Peter Williams and Nelson, {Randall W.}",

year = "2002",

language = "English (US)",

pages = "269--270",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - GEN

T1 - High throughput characterization of hemoglobin from neonates using MALDI-TOF MS

AU - Kiernan, Urban A.

AU - Williams, Peter

AU - Nelson, Randall W.

PY - 2002

Y1 - 2002

N2 - MALDI-TOF targets were used to create a high throughput platform capable of characterizing hemoglobin from neonates at a rate of ∼ 100 samples per hour. Ninety-six neonate dried blood blot samples were collected and isolated in individual wells of a 96-well titer plate and robotically processed in parallel acidified hemoglobin solutions. The intact chains of 96 hemoglobin samples were screened. The absence of an additional signal, either split or shifted by ∼-30 Da, indicates that the mutation was present in the first eight residues of the β-chain and due to the β6Glu Val substitution.

AB - MALDI-TOF targets were used to create a high throughput platform capable of characterizing hemoglobin from neonates at a rate of ∼ 100 samples per hour. Ninety-six neonate dried blood blot samples were collected and isolated in individual wells of a 96-well titer plate and robotically processed in parallel acidified hemoglobin solutions. The intact chains of 96 hemoglobin samples were screened. The absence of an additional signal, either split or shifted by ∼-30 Da, indicates that the mutation was present in the first eight residues of the β-chain and due to the β6Glu Val substitution.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442697925&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442697925

SP - 269

EP - 270

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

High throughput characterization of hemoglobin from neonates using MALDI-TOF MS

Abstract

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ASJC Scopus subject areas

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