High-throughput affinity mass spectrometry.

Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, Randall W. Nelson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biological sample. Information regarding such protein diversity is normally lost in classical proteomic or immunoassay analyses. This chapter presents a step-by-step description of high-throughput AMS in the population proteomic screening of the human plasma protein cystatin C.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume328
DOIs
StatePublished - 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'High-throughput affinity mass spectrometry.'. Together they form a unique fingerprint.

Cite this