High-throughput affinity mass spectrometry.

Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, Randall W. Nelson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biological sample. Information regarding such protein diversity is normally lost in classical proteomic or immunoassay analyses. This chapter presents a step-by-step description of high-throughput AMS in the population proteomic screening of the human plasma protein cystatin C.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume328
StatePublished - 2006

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Mass Spectrometry
Proteomics
Proteins
Cystatin C
Protein C
Immunoassay
Blood Proteins
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kiernan, U. A., Nedelkov, D., Niederkofler, E. E., Tubbs, K. A., & Nelson, R. W. (2006). High-throughput affinity mass spectrometry. Methods in molecular biology (Clifton, N.J.), 328, 141-150.

High-throughput affinity mass spectrometry. / Kiernan, Urban A.; Nedelkov, Dobrin; Niederkofler, Eric E.; Tubbs, Kemmons A.; Nelson, Randall W.

In: Methods in molecular biology (Clifton, N.J.), Vol. 328, 2006, p. 141-150.

Research output: Contribution to journalArticle

Kiernan, UA, Nedelkov, D, Niederkofler, EE, Tubbs, KA & Nelson, RW 2006, 'High-throughput affinity mass spectrometry.', Methods in molecular biology (Clifton, N.J.), vol. 328, pp. 141-150.
Kiernan UA, Nedelkov D, Niederkofler EE, Tubbs KA, Nelson RW. High-throughput affinity mass spectrometry. Methods in molecular biology (Clifton, N.J.). 2006;328:141-150.
Kiernan, Urban A. ; Nedelkov, Dobrin ; Niederkofler, Eric E. ; Tubbs, Kemmons A. ; Nelson, Randall W. / High-throughput affinity mass spectrometry. In: Methods in molecular biology (Clifton, N.J.). 2006 ; Vol. 328. pp. 141-150.
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