High resolution surface plasmon resonance spectroscopy

Nongjian Tao, S. Boussaad, W. L. Huang, R. A. Arechabaleta, J. D'Agnese

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A method for detecting surface plasmon resonance with high resolution (∼ 10-5 degrees or ∼ 10-8 refractive index units) and fast response time (1 μs) is described. In the method, light is focused through a prism onto a metal film on which molecules to be detected are adsorbed. The total internal reflection of the incident light is collected with a bicell photodetector instead of a single cell or an array of photodetectors that are widely used in previous works. The ratio of the differential signal to the sum signal of the bicell photodetector provides an accurate measurement of shift in surface plasmon resonance angle caused by the adsorption of molecules onto the metal films or by conformational changes in the adsorbed molecules. Using the method, we have studied subtle conformational changes in redox protein, cytochrome c, due to an electron transfer reaction.

Original languageEnglish (US)
Pages (from-to)4656-4660
Number of pages5
JournalReview of Scientific Instruments
Volume70
Issue number12
StatePublished - Dec 1999
Externally publishedYes

Fingerprint

Surface plasmon resonance
Photodetectors
surface plasmon resonance
photometers
Spectroscopy
metal films
Molecules
high resolution
spectroscopy
Proteins
molecules
cytochromes
Prisms
Metals
prisms
Refractive index
electron transfer
refractivity
proteins
Adsorption

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Instrumentation

Cite this

Tao, N., Boussaad, S., Huang, W. L., Arechabaleta, R. A., & D'Agnese, J. (1999). High resolution surface plasmon resonance spectroscopy. Review of Scientific Instruments, 70(12), 4656-4660.

High resolution surface plasmon resonance spectroscopy. / Tao, Nongjian; Boussaad, S.; Huang, W. L.; Arechabaleta, R. A.; D'Agnese, J.

In: Review of Scientific Instruments, Vol. 70, No. 12, 12.1999, p. 4656-4660.

Research output: Contribution to journalArticle

Tao, N, Boussaad, S, Huang, WL, Arechabaleta, RA & D'Agnese, J 1999, 'High resolution surface plasmon resonance spectroscopy', Review of Scientific Instruments, vol. 70, no. 12, pp. 4656-4660.
Tao N, Boussaad S, Huang WL, Arechabaleta RA, D'Agnese J. High resolution surface plasmon resonance spectroscopy. Review of Scientific Instruments. 1999 Dec;70(12):4656-4660.
Tao, Nongjian ; Boussaad, S. ; Huang, W. L. ; Arechabaleta, R. A. ; D'Agnese, J. / High resolution surface plasmon resonance spectroscopy. In: Review of Scientific Instruments. 1999 ; Vol. 70, No. 12. pp. 4656-4660.
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